MRPL4

Protein identityUniProt · HPA

UniProt accession

Q9BYD3

Gene

MRPL4

Ensembl

ENSG00000105364

Chromosome

19

Canonical length

311 aa

Protein class

Predicted intracellular proteins, Ribosomal proteins

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

311 residues, UniProt reviewed canonical sequence.

>Q9BYD3|MRPL4
     1  MLQFVRAGAR AWLRPTGSQG LSSLAEEAAR ATENPEQVAS EGLPEPVLRK VELPVPTHRR
    61  PVQAWVESLR GFEQERVGLA DLHPDVFATA PRLDILHQVA MWQKNFKRIS YAKTKTRAEV
   121  RGGGRKPWPQ KGTGRARHGS IRSPLWRGGG VAHGPRGPTS YYYMLPMKVR ALGLKVALTV
   181  KLAQDDLHIM DSLELPTGDP QYLTELAHYR RWGDSVLLVD LTHEEMPQSI VEATSRLKTF
   241  NLIPAVGLNV HSMLKHQTLV LTLPTVAFLE DKLLWQDSRY RPLYPFSLPY SDFPRPLPHA
   301  TQGPAATPYH C

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPL4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.41

Highest tissue expression

97 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MRPL4.

Disease | ImmuneIEDB

Conditions an epitope on MRPL4 was assayed in.

• human immunodeficiency virus infectious disease T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.44

gnomAD pLI

0

gnomAD missense Z

0.37

DepMap mean gene effect

-0.62

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mitochondrial translation

Molecular functions

• RNA binding
• structural constituent of ribosome

Cellular components

• mitochondrial inner membrane
• mitochondrial large ribosomal subunit
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Large ribosomal subunit protein uL4
• Large ribosomal subunit protein uL4 domain superfamily
• Ribosomal protein L4/L1 family
• Large ribosomal subunit protein uL4-like

KeywordsUniProt

• Methylation
• Mitochondrion
• Ribonucleoprotein
• Ribosomal protein