Seroatlas · Human Serome Atlas

MRPL35

Large ribosomal subunit protein bL35m

Also known as: RM35_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NZE8
Gene
MRPL35
Ensembl
ENSG00000132313
Chromosome
2
Canonical length
188 aa
Protein class
Predicted intracellular proteins, Ribosomal proteins

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p, 10q, and Xp. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

188 residues, UniProt reviewed canonical sequence.

>Q9NZE8|MRPL35
     1  MAASAFAGAV RAASGILRPL NILASSTYRN CVKNASLISA LSTGRFSHIQ TPVVSSTPRL
    61  TTSERNLTCG HTSVILNRMA PVLPSVLKLP VRSLTYFSAR KGKRKTVKAV IDRFLRLHCG
   121  LWVRRKAGYK KKLWKKTPAR KKRLREFVFC NKTQSKLLDK MTTSFWKRRN WYVDDPYQKY
   181  HDRTNLKV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPL35 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.56
Highest tissue expression
106 nTPM

Expression across tissuesHPA

Tissue

  • tongue: 106 nTPM
  • heart muscle: 79 nTPM
  • skeletal muscle: 65 nTPM
  • rectum: 55 nTPM
  • duodenum: 54 nTPM
  • colon: 52 nTPM

Single-cell type

  • parietal cells: 145 nCPM
  • hofbauer cells: 85 nCPM
  • esophageal suprabasal cells: 74 nCPM
  • esophageal basal cells: 72 nCPM
  • erythrocyte progenitors: 72 nCPM
  • enteric transient amplifying cells: 68 nCPM

Immune cell

  • non-classical monocyte: 51 nTPM
  • intermediate monocyte: 49 nTPM
  • memory B-cell: 45 nTPM
  • plasmacytoid DC: 42 nTPM
  • myeloid DC: 42 nTPM
  • naive B-cell: 42 nTPM

Brain region

  • cerebral cortex: 39 nTPM
  • hypothalamus: 32 nTPM
  • basal ganglia: 32 nTPM
  • thalamus: 32 nTPM
  • cerebellum: 31 nTPM
  • pons: 30 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.29
gnomAD pLI
0
gnomAD missense Z
0.06
DepMap mean gene effect
-0.57
DepMap dependency class
common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Large ribosomal subunit protein bL35m
  • Large ribosomal subunit protein bL35-like
  • Large ribosomal subunit protein bL35 superfamily
  • Ribosomal protein L35

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MRPL35 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MRPL35 as an antibody target. Whether an autoantibody or antibody against MRPL35 could matter depends on whether native MRPL35 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MRPL35 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MRPL35 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MRPL35. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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