Seroatlas · Human Serome Atlas

MPST

3-mercaptopyruvate sulfurtransferase

Also known as: MST, THTM_HUMAN, TST2, TUM1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P25325
Gene
MPST
Ensembl
ENSG00000128309
Chromosome
22
Canonical length
297 aa
Protein class
Disease related genes, Enzymes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins
Subcellular location
Mitochondria,Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

297 residues, UniProt reviewed canonical sequence.

>P25325|MPST
     1  MASPQLCRAL VSAQWVAEAL RAPRAGQPLQ LLDASWYLPK LGRDARREFE ERHIPGAAFF
    61  DIDQCSDRTS PYDHMLPGAE HFAEYAGRLG VGAATHVVIY DASDQGLYSA PRVWWMFRAF
   121  GHHAVSLLDG GLRHWLRQNL PLSSGKSQPA PAEFRAQLDP AFIKTYEDIK ENLESRRFQV
   181  VDSRATGRFR GTEPEPRDGI EPGHIPGTVN IPFTDFLSQE GLEKSPEEIR HLFQEKKVDL
   241  SKPLVATCGS GVTACHVALG AYLCGKPDVP IYDGSWVEWY MRARPEDVIS EGRGKTH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MPST can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
371 nTPM

Expression across tissuesHPA

Tissue

  • liver: 371 nTPM
  • pancreas: 101 nTPM
  • duodenum: 100 nTPM
  • colon: 96 nTPM
  • small intestine: 88 nTPM
  • esophagus: 87 nTPM

Single-cell type

  • enterocytes: 1,170 nCPM
  • esophageal apical cells: 675 nCPM
  • hepatocytes: 650 nCPM
  • colonocytes: 517 nCPM
  • enteric transient amplifying cells: 380 nCPM
  • esophageal suprabasal cells: 348 nCPM

Immune cell

  • myeloid DC: 104 nTPM
  • NK-cell: 88 nTPM
  • classical monocyte: 72 nTPM
  • eosinophil: 55 nTPM
  • basophil: 49 nTPM
  • T-reg: 44 nTPM

Brain region

  • white matter: 55 nTPM
  • medulla oblongata: 53 nTPM
  • basal ganglia: 49 nTPM
  • thalamus: 47 nTPM
  • choroid plexus: 45 nTPM
  • cerebellum: 43 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.34
gnomAD pLI
0
gnomAD missense Z
1.21
DepMap mean gene effect
-0.02
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MPST as an antibody target. Whether an autoantibody or antibody against MPST could matter depends on whether native MPST is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MPST is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MPST as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MPST. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...