Seroatlas · Human Serome Atlas

MIXL1

Homeobox protein MIXL1

Also known as: MILD1, MIXL, MIXL1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H2W2
Gene: MIXL1
Ensembl: ENSG00000185155
Chromosome: 1
Canonical length: 232 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Enables RNA polymerase II-specific DNA-binding transcription factor binding activity and sequence-specific double-stranded DNA binding activity. Predicted to be involved in several processes, including endodermal cell differentiation; negative regulation of hematopoietic progenitor cell differentiation; and positive regulation of mesoderm development. Predicted to act upstream of or within cell migration involved in gastrulation and hemopoiesis. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

232 residues, UniProt reviewed canonical sequence.

>Q9H2W2|MIXL1
     1  MATAESRALQ FAEGAAFPAY RAPHAGGALL PPPSPAAALL PAPPAGPGPA TFAGFLGRDP
    61  GPAPPPPASL GSPAPPKGAA APSASQRRKR TSFSAEQLQL LELVFRRTRY PDIHLRERLA
   121  ALTLLPESRI QVWFQNRRAK SRRQSGKSFQ PLARPEIILN HCAPGTETKC LKPQLPLEVD
   181  VNCLPEPNGV GGGISDSSSQ GQNFETCSPL SEDIGSKLDS WEEHIFSAFG NF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MIXL1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.63
Highest tissue expression: 3.2 nTPM

Expression across tissuesHPA

Tissue

tonsil: 3.2 nTPM
lymph node: 2.3 nTPM
duodenum: 1.2 nTPM
appendix: 1 nTPM
cerebellum: 1 nTPM
spleen: 0.9 nTPM

Single-cell type

oocytes: 23 nCPM
plasma cells: 16 nCPM
hematopoietic stem cells: 3.4 nCPM
undifferentiated spermatogonia: 2.9 nCPM
medullary thymic epithelial cells: 2.4 nCPM
thymic myoid cells: 1.4 nCPM

Immune cell

basophil: 49 nTPM
NK-cell: 19 nTPM
non-classical monocyte: 19 nTPM
neutrophil: 19 nTPM
eosinophil: 13 nTPM
MAIT T-cell: 12 nTPM

Brain region

cerebellum: 3.4 nTPM
white matter: 1.7 nTPM
pons: 1.6 nTPM
amygdala: 1.5 nTPM
cerebral cortex: 1.5 nTPM
medulla oblongata: 1.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.56
gnomAD pLI: 0.02
gnomAD missense Z: 0.43
DepMap mean gene effect: -0.13
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MIXL1 as an antibody target. Whether an autoantibody or antibody against MIXL1 could matter depends on whether native MIXL1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MIXL1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MIXL1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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