Seroatlas · Human Serome Atlas

MIB2

E3 ubiquitin-protein ligase MIB2

Also known as: FLJ39787, MIB2_HUMAN, skeletrophin, ZZANK1, ZZZ5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96AX9
Gene
MIB2
Ensembl
ENSG00000197530
Chromosome
1
Canonical length
955 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion. [provided by RefSeq, Mar 2017]

Canonical amino-acid sequenceUniProt

955 residues, UniProt reviewed canonical sequence.

>Q96AX9|MIB2
     1  MDPDPQAGVQ VGMRVVRGVD WKWGQQDGGE GGVGTVVELG RHGSPSTPDR TVVVQWDQGT
    61  RTNYRAGYQG AHDLLLYDNA QIGVRHPNII CDCCKKHGLR GMRWKCRVCL DYDLCTQCYM
   121  HNKHELAHAF DRYETAHSRP VTLSPRQGLP RIPLRGIFQG AKVVRGPDWE WGSQDGGEGK
   181  PGRVVDIRGW DVETGRSVAS VTWADGTTNV YRVGHKGKVD LKCVGEAAGG FYYKDHLPRL
   241  GKPAELQRRV SADSQPFQHG DKVKCLLDTD VLREMQEGHG GWNPRMAEFI GQTGTVHRIT
   301  DRGDVRVQFN HETRWTFHPG ALTKHHSFWV GDVVRVIGDL DTVKRLQAGH GEWTDDMAPA
   361  LGRVGKVVKV FGDGNLRVAV AGQRWTFSPS CLVAYRPEED ANLDVAERAR ENKSSLSVAL
   421  DKLRAQKSDP EHPGRLVVEV ALGNAARALD LLRRRPEQVD TKNQGRTALQ VAAYLGQVEL
   481  IRLLLQARAG VDLPDDEGNT ALHYAALGNQ PEATRVLLSA GCRADAINST QSTALHVAVQ
   541  RGFLEVVRAL CERGCDVNLP DAHSDTPLHS AISAGTGASG IVEVLTEVPN IDVTATNSQG
   601  FTLLHHASLK GHALAVRKIL ARARQLVDAK KEDGFTALHL AALNNHREVA QILIREGRCD
   661  VNVRNRKLQS PLHLAVQQAH VGLVPLLVDA GCSVNAEDEE GDTALHVALQ RHQLLPLVAD
   721  GAGGDPGPLQ LLSRLQASGL PGSAELTVGA AVACFLALEG ADVSYTNHRG RSPLDLAAEG
   781  RVLKALQGCA QRFRERQAGG GAAPGPRQTL GTPNTVTNLH VGAAPGPEAA ECLVCSELAL
   841  LVLFSPCQHR TVCEECARRM KKCIRCQVVV SKKLRPDGSE VASAAPAPGP PRQLVEELQS
   901  RYRQMEERIT CPICIDSHIR LVFQCGHGAC APCGSALSAC PICRQPIRDR IQIFV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MIB2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.29
Highest tissue expression
47 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 47 nTPM
  • esophagus: 19 nTPM
  • cerebral cortex: 19 nTPM
  • hippocampal formation: 18 nTPM
  • skin: 18 nTPM
  • basal ganglia: 18 nTPM

Single-cell type

  • late spermatids: 409 nCPM
  • esophageal basal cells: 217 nCPM
  • gastric progenitor cells: 154 nCPM
  • esophageal suprabasal cells: 150 nCPM
  • basal keratinocytes: 126 nCPM
  • suprabasal keratinocytes: 108 nCPM

Immune cell

  • plasmacytoid DC: 7.3 nTPM
  • NK-cell: 6.3 nTPM
  • memory CD8 T-cell: 3.5 nTPM
  • naive B-cell: 3.4 nTPM
  • MAIT T-cell: 3.2 nTPM
  • gdT-cell: 3.1 nTPM

Brain region

  • medulla oblongata: 29 nTPM
  • cerebral cortex: 27 nTPM
  • basal ganglia: 25 nTPM
  • hippocampal formation: 25 nTPM
  • hypothalamus: 25 nTPM
  • white matter: 24 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.62
gnomAD pLI
0
gnomAD missense Z
0.62
DepMap mean gene effect
-0.13
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MIB2 as an antibody target. Whether an autoantibody or antibody against MIB2 could matter depends on whether native MIB2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MIB2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MIB2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MIB2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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