Seroatlas · Human Serome Atlas

MGAT5

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Also known as: GNT-V, MGAT5A, MGT5A_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q09328
Gene: MGAT5
Ensembl: ENSG00000152127
Chromosome: 2
Canonical length: 741 aa
Protein class: Enzymes, Metabolic proteins, Predicted membrane proteins
Secretome location: Intracellular and membrane

OverviewNCBI Gene

The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]

Canonical amino-acid sequenceUniProt

741 residues, UniProt reviewed canonical sequence.

>Q09328|MGAT5
     1  MALFTPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI LDLSKRYIKA
    61  LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL ESKVDNLVVN GTGTNSTNST
   121  TAVPSLVALE KINVADIING AQEKCVLPPM DGYPHCEGKI KWMKDMWRSD PCYADYGVDG
   181  STCSFFIYLS EVENWCPHLP WRAKNPYEEA DHNSLAEIRT DFNILYSMMK KHEEFRWMRL
   241  RIRRMADAWI QAIKSLAEKQ NLEKRKRKKV LVHLGLLTKE SGFKIAETAF SGGPLGELVQ
   301  WSDLITSLYL LGHDIRISAS LAELKEIMKK VVGNRSGCPT VGDRIVELIY IDIVGLAQFK
   361  KTLGPSWVHY QCMLRVLDSF GTEPEFNHAN YAQSKGHKTP WGKWNLNPQQ FYTMFPHTPD
   421  NSFLGFVVEQ HLNSSDIHHI NEIKRQNQSL VYGKVDSFWK NKKIYLDIIH TYMEVHATVY
   481  GSSTKNIPSY VKNHGILSGR DLQFLLRETK LFVGLGFPYE GPAPLEAIAN GCAFLNPKFN
   541  PPKSSKNTDF FIGKPTLREL TSQHPYAEVF IGRPHVWTVD LNNQEEVEDA VKAILNQKIE
   601  PYMPYEFTCE GMLQRINAFI EKQDFCHGQV MWPPLSALQV KLAEPGQSCK QVCQESQLIC
   661  EPSFFQHLNK DKDMLKYKVT CQSSELAKDI LVPSFDPKNK HCVFQGDLLL FSCAGAHPRH
   721  QRVCPCRDFI KGQVALCKDC L

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MGAT5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 37 nTPM

Expression across tissuesHPA

Tissue

heart muscle: 37 nTPM
tonsil: 24 nTPM
lymph node: 23 nTPM
spinal cord: 23 nTPM
fallopian tube: 18 nTPM
thymus: 18 nTPM

Single-cell type

podocytes: 1,513 nCPM
endometrial glandular cells: 1,425 nCPM
b-cells: 1,123 nCPM
innate lymphoid cells: 687 nCPM
respiratory ionocytes: 668 nCPM
thymocytes: 660 nCPM

Immune cell

naive B-cell: 3.7 nTPM
memory B-cell: 3 nTPM
memory CD4 T-cell: 1.5 nTPM
MAIT T-cell: 1.3 nTPM
NK-cell: 1.3 nTPM
neutrophil: 1.1 nTPM

Brain region

midbrain: 71 nTPM
hypothalamus: 70 nTPM
pons: 69 nTPM
cerebellum: 64 nTPM
medulla oblongata: 60 nTPM
white matter: 55 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.18
gnomAD pLI: 1
gnomAD missense Z: 2.56
DepMap mean gene effect: 0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MGAT5 as an antibody target. Whether an autoantibody or antibody against MGAT5 could matter depends on whether native MGAT5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MGAT5 is annotated as secreted, so native MGAT5 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label MGAT5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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