Seroatlas · Human Serome Atlas

MFNG

Beta-1,3-N-acetylglucosaminyltransferase manic fringe

Also known as: MFNG_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O00587
Gene: MFNG
Ensembl: ENSG00000100060
Chromosome: 22
Canonical length: 321 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Vesicles
Secretome location: Intracellular and membrane

OverviewNCBI Gene

This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the LFNG (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene may control Notch signaling in claudin-low breast cancer. [provided by RefSeq, May 2018]

Canonical amino-acid sequenceUniProt

321 residues, UniProt reviewed canonical sequence.

>O00587|MFNG
     1  MQCRLPRGLA GALLTLLCMG LLCLRYHLNL SPQRVQGTPE LSQPNPGPPK LQLHDVFIAV
    61  KTTRAFHRLR LELLLDTWVS RTREQTFVFT DSPDKGLQER LGSHLVVTNC SAEHSHPALS
   121  CKMAAEFDTF LASGLRWFCH VDDDNYVNPR ALLQLLRAFP LARDVYVGRP SLNRPIHASE
   181  PQPHNRTRLV QFWFATGGAG FCINRKLALK MAPWASGSRF MDTSALIRLP DDCTMGYIIE
   241  CKLGGRLQPS PLFHSHLETL QLLRTAQLPE QVTLSYGVFE GKLNVIKLQG PFSPEEDPSR
   301  FRSLHCLLYP DTPWCPQLGA R

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MFNG can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 65 nTPM

Expression across tissuesHPA

Tissue

lymph node: 65 nTPM
spleen: 58 nTPM
thymus: 47 nTPM
tonsil: 40 nTPM
bone marrow: 38 nTPM
appendix: 35 nTPM

Single-cell type

megakaryocytes: 173 nCPM
platelets: 138 nCPM
megakaryocyte progenitors: 66 nCPM
lymphatic endothelial cells: 47 nCPM
nk-cells: 42 nCPM
vascular endothelial cells: 40 nCPM

Immune cell

eosinophil: 388 nTPM
total PBMC: 314 nTPM
T-reg: 285 nTPM
naive CD4 T-cell: 244 nTPM
memory CD4 T-cell: 223 nTPM
non-classical monocyte: 220 nTPM

Brain region

pons: 36 nTPM
medulla oblongata: 23 nTPM
thalamus: 17 nTPM
midbrain: 16 nTPM
white matter: 15 nTPM
spinal cord: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.56
gnomAD pLI: 0
gnomAD missense Z: 0.3
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MFNG as an antibody target. Whether an autoantibody or antibody against MFNG could matter depends on whether native MFNG is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MFNG is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MFNG as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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