Seroatlas · Human Serome Atlas

MFI

Protein MFI

Also known as: C11orf65, MFI_HUMAN, MGC33948

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NCR3
Gene: MFI
Ensembl: ENSG00000166323
Chromosome: 11
Canonical length: 313 aa
Protein class: Predicted intracellular proteins
Quaternary structure: Homodimer

OverviewNCBI Gene

Predicted to be involved in negative regulation of mitochondrial fission and negative regulation of protein targeting to mitochondrion. Predicted to be located in cytosol and mitochondrial outer membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

313 residues, UniProt reviewed canonical sequence.

>Q8NCR3|MFI
     1  MPWKEESEFT KQDKAARVIQ QAWKSFLNVA IFQHFKSLID LRRQGEPRQI VKYINPKEAE
    61  LLDAAAGIHV RFRLGGVKFP PDIYYKIFTH RPIEDLCANS PRNYAKLPAK HTSHNKNDHL
   121  QEEDHSGWYH RIENNGWRPV SDTFWLSTDG MVVEDKKESE FHFSKLKRRQ DLEKKRKLRK
   181  IEWMRQMYYS GSLEAKSTHH ETLGLIHTAT KGLIRAFEDG GIDSVMEWEV DEVLNWTNTL
   241  NFDEYIASWK EIATSNSSAN FKGFRFNQAQ KNIYNYGGDI SKMQMGIPDD TYYENVYQEP
   301  NVTRLTPDST YGL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MFI can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 47 nTPM

Expression across tissuesHPA

Tissue

testis: 47 nTPM
retina: 2.6 nTPM
liver: 2.4 nTPM
epididymis: 2.3 nTPM
kidney: 1.9 nTPM
thyroid gland: 1.6 nTPM

Single-cell type

early spermatids: 925 nCPM
late primary spermatocytes: 679 nCPM
early primary spermatocytes: 250 nCPM
epicardial cells: 227 nCPM
cardiomyocytes: 194 nCPM
rod photoreceptor cells: 112 nCPM

Immune cell

MAIT T-cell: 0.5 nTPM
basophil: 0.4 nTPM
plasmacytoid DC: 0.4 nTPM
intermediate monocyte: 0.3 nTPM
naive B-cell: 0.2 nTPM
naive CD4 T-cell: 0.2 nTPM

Brain region

white matter: 6.6 nTPM
basal ganglia: 5.1 nTPM
medulla oblongata: 5.1 nTPM
spinal cord: 4.7 nTPM
thalamus: 4.4 nTPM
midbrain: 4.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.75
gnomAD pLI: 0
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MFI in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MFI as an antibody target. Whether an autoantibody or antibody against MFI could matter depends on whether native MFI is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MFI is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MFI as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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