Seroatlas · Human Serome Atlas

METTL4

N(6)-adenine-specific methyltransferase METTL4

Also known as: FLJ23017, HsT661, METL4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8N3J2
Gene
METTL4
Ensembl
ENSG00000101574
Chromosome
18
Canonical length
472 aa
Protein class
Enzymes, Predicted intracellular proteins
Subcellular location
Mitochondria

OverviewNCBI Gene

Enables RNA methyltransferase activity and site-specific DNA-methyltransferase (adenine-specific) activity. Involved in regulation of primary metabolic process and snRNA (adenine-N6)-methylation. Located in cytosol; mitochondrial matrix; and nucleus. Part of RNA N6-methyladenosine methyltransferase complex and oxidoreductase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

472 residues, UniProt reviewed canonical sequence.

>Q8N3J2|METTL4
     1  MSVVHQLSAG WLLDHLSFIN KINYQLHQHH EPCCRKKEFT TSVHFESLQM DSVSSSGVCA
    61  AFIASDSSTK PENDDGGNYE MFTRKFVFRP ELFDVTKPYI TPAVHKECQQ SNEKEDLMNG
   121  VKKEISISII GKKRKRCVVF NQGELDAMEY HTKIRELILD GSLQLIQEGL KSGFLYPLFE
   181  KQDKGSKPIT LPLDACSLSE LCEMAKHLPS LNEMEHQTLQ LVEEDTSVTE QDLFLRVVEN
   241  NSSFTKVITL MGQKYLLPPK SSFLLSDISC MQPLLNYRKT FDVIVIDPPW QNKSVKRSNR
   301  YSYLSPLQIQ QIPIPKLAAP NCLLVTWVTN RQKHLRFIKE ELYPSWSVEV VAEWHWVKIT
   361  NSGEFVFPLD SPHKKPYEGL ILGRVQEKTA LPLRNADVNV LPIPDHKLIV SVPCTLHSHK
   421  PPLAEVLKDY IKPDGEYLEL FARNLQPGWT SWGNEVLKFQ HVDYFIAVES GS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against METTL4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.34
Highest tissue expression
11 nTPM

Expression across tissuesHPA

Tissue

  • lymph node: 11 nTPM
  • tonsil: 10 nTPM
  • thymus: 9.7 nTPM
  • thyroid gland: 8.3 nTPM
  • spleen: 7.7 nTPM
  • appendix: 7.4 nTPM

Single-cell type

  • pituicytes/fscs: 37 nCPM
  • early primary spermatocytes: 37 nCPM
  • b-cells: 35 nCPM
  • oligodendrocytes: 31 nCPM
  • t-cells: 31 nCPM
  • lymphatic endothelial cells: 31 nCPM

Immune cell

  • basophil: 19 nTPM
  • neutrophil: 10 nTPM
  • T-reg: 8.8 nTPM
  • memory CD8 T-cell: 8.5 nTPM
  • naive CD4 T-cell: 8.5 nTPM
  • naive CD8 T-cell: 7.9 nTPM

Brain region

  • white matter: 13 nTPM
  • pons: 11 nTPM
  • medulla oblongata: 11 nTPM
  • cerebral cortex: 11 nTPM
  • spinal cord: 10 nTPM
  • basal ganglia: 9.8 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about METTL4.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 72 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.95
gnomAD pLI
0
gnomAD missense Z
0.39
DepMap mean gene effect
0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads METTL4 as an antibody target. Whether an autoantibody or antibody against METTL4 could matter depends on whether native METTL4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

METTL4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label METTL4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/METTL4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...