Seroatlas · Human Serome Atlas

METTL25B

Methyltransferase-like protein 25B

Also known as: C1orf66, CGI-41, MT25B_HUMAN, RRNAD1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96FB5
Gene: METTL25B
Ensembl: ENSG00000143303
Chromosome: 1
Canonical length: 475 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoli,Cytosol

OverviewNCBI Gene

Predicted to enable rRNA (adenine-N6,N6-)-dimethyltransferase activity. Predicted to be involved in rRNA methylation. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

475 residues, UniProt reviewed canonical sequence.

>Q96FB5|METTL25B
     1  MPGISARGLS HEGRKQLAVN LTRVLALYRS ILDAYIIEFF TDNLWDTLPC SWQEALDGLK
    61  PPQLATMLLG MPGEGEVVRY RSVWPLTLLA LKSTACALAF TRMPGFQTPS EFLENPSQSS
   121  RLTAPFRKHV RPKKQHEIRR LGELVKKLSD FTGCTQVVDV GSGQGHLSRF MALGLGLMVK
   181  SIEGDQRLVE RAQRLDQELL QALEKEEKRN PQVVQTSPRH SPHHVVRWVD PTALCEELLL
   241  PLENPCQGRA RLLLTGLHAC GDLSVALLRH FSCCPEVVAL ASVGCCYMKL SDPGGYPLSQ
   301  WVAGLPGYEL PYRLREGACH ALEEYAERLQ KAGPGLRTHC YRAALETVIR RARPELRRPG
   361  VQGIPRVHEL KIEEYVQRGL QRVGLDPQLP LNLAALQAHV AQENRVVAFF SLALLLAPLV
   421  ETLILLDRLL YLQEQGFHAE LLPIFSPELS PRNLVLVATK MPLGQALSVL ETEDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against METTL25B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 28 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 28 nTPM
thyroid gland: 28 nTPM
skin: 27 nTPM
liver: 27 nTPM
fallopian tube: 27 nTPM
pancreas: 26 nTPM

Single-cell type

early spermatids: 129 nCPM
epicardial cells: 66 nCPM
syncytiotrophoblasts: 53 nCPM
cholangiocytes: 49 nCPM
enterocytes: 48 nCPM
late primary spermatocytes: 45 nCPM

Immune cell

T-reg: 5.1 nTPM
basophil: 4.1 nTPM
neutrophil: 4 nTPM
NK-cell: 4 nTPM
MAIT T-cell: 3.6 nTPM
gdT-cell: 3.5 nTPM

Brain region

white matter: 33 nTPM
medulla oblongata: 31 nTPM
choroid plexus: 30 nTPM
basal ganglia: 28 nTPM
cerebellum: 26 nTPM
pons: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.21
gnomAD pLI: 0
DepMap mean gene effect: -0.05
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

N6-)-dimethyltransferase activity
rRNA (adenine-N6

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads METTL25B as an antibody target. Whether an autoantibody or antibody against METTL25B could matter depends on whether native METTL25B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

METTL25B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label METTL25B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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