MEST

Protein identityUniProt · HPA

UniProt accession

Q5EB52

Gene

MEST

Ensembl

ENSG00000106484

Chromosome

7

Canonical length

335 aa

Protein class

Predicted intracellular proteins

Subcellular location

Golgi apparatus,Cytosol

Secretome location

Intracellular and membrane

OverviewNCBI Gene

This gene encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15. [provided by RefSeq, Dec 2011]

Canonical amino-acid sequenceUniProt

335 residues, UniProt reviewed canonical sequence.

>Q5EB52|MEST
     1  MVRRDRLRRM REWWVQVGLL AVPLLAAYLH IPPPQLSPAL HSWKSSGKFF TYKGLRIFYQ
    61  DSVGVVGSPE IVVLLHGFPT SSYDWYKIWE GLTLRFHRVI ALDFLGFGFS DKPRPHHYSI
   121  FEQASIVEAL LRHLGLQNRR INLLSHDYGD IVAQELLYRY KQNRSGRLTI KSLCLSNGGI
   181  FPETHRPLLL QKLLKDGGVL SPILTRLMNF FVFSRGLTPV FGPYTRPSES ELWDMWAGIR
   241  NNDGNLVIDS LLQYINQRKK FRRRWVGALA SVTIPIHFIY GPLDPVNPYP EFLELYRKTL
   301  PRSTVSILDD HISHYPQLED PMGFLNAYMG FINSF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MEST can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

3

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

725 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MEST.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 52 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Childhood-onset schizophrenia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.47

gnomAD pLI

0.4

gnomAD missense Z

2.36

DepMap mean gene effect

0

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• fat cell differentiation
• mesoderm development
• negative regulation of canonical Wnt signaling pathway
• negative regulation of glycoprotein biosynthetic process
• negative regulation of protein localization to plasma membrane
• neuron development
• radial glia-guided pyramidal neuron migration
• regulation of lipid storage
• regulation of neurogenesis
• response to retinoic acid

Molecular functions

• hydrolase activity

Cellular components

• endoplasmic reticulum
• endoplasmic reticulum membrane
• extracellular exosome

Protein domainsUniProt · Pfam · InterPro

• Alpha/beta hydrolase fold-1
• Epoxide hydrolase-like
• Alpha/Beta hydrolase fold
• AB hydrolase superfamily
• alpha/beta hydrolase fold

KeywordsUniProt

• Endoplasmic reticulum
• Glycoprotein
• Hydrolase
• Membrane
• Neurogenesis
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of MEST in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• LRP6