Seroatlas · Human Serome Atlas

MARCHF9

E3 ubiquitin-protein ligase MARCHF9

Also known as: FLJ36578, MARCH9, MARH9_HUMAN, RNF179

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q86YJ5
Gene
MARCHF9
Ensembl
ENSG00000139266
Chromosome
12
Canonical length
346 aa
Protein class
Enzymes, Metabolic proteins, Predicted membrane proteins
Quaternary structure
Homodimer

OverviewNCBI Gene

MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]).[supplied by OMIM, Apr 2010]

Canonical amino-acid sequenceUniProt

346 residues, UniProt reviewed canonical sequence.

>Q86YJ5|MARCHF9
     1  MLKSRLRMFL NELKLLVLTG GGRPRAEPQP RGGRGGGCGW APFAGCSTRD GDGDEEEYYG
    61  SEPRARGLAG DKEPRAGPLP PPAPPLPPPG ALDALSLSSS LDSGLRTPQC RICFQGPEQG
   121  ELLSPCRCDG SVRCTHQPCL IRWISERGSW SCELCYFKYQ VLAISTKNPL QWQAISLTVI
   181  EKVQIAAIVL GSLFLVASIS WLIWSSLSPS AKWQRQDLLF QICYGMYGFM DVVCIGLIIH
   241  EGSSVYRIFK RWQAVNQQWK VLNYDKTKDI GGDAGGGTAG KSGPRNSRTG PTSGATSRPP
   301  AAQRMRTLLP QRCGYTILHL LGQLRPPDAR SSSHSGREVV MRVTTV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
2
Mean surface accessibility (rSASA)
0.53
Highest tissue expression
38 nTPM

Expression across tissuesHPA

Tissue

  • retina: 38 nTPM
  • cerebellum: 34 nTPM
  • pituitary gland: 34 nTPM
  • spleen: 28 nTPM
  • kidney: 27 nTPM
  • thyroid gland: 25 nTPM

Single-cell type

  • rod photoreceptor cells: 88 nCPM
  • cone photoreceptor cells: 75 nCPM
  • pdcs: 70 nCPM
  • müller glia: 51 nCPM
  • retinal bipolar cells: 50 nCPM
  • alveolar cells type 1: 40 nCPM

Immune cell

  • plasmacytoid DC: 9.8 nTPM
  • naive B-cell: 5.8 nTPM
  • memory CD4 T-cell: 5.1 nTPM
  • MAIT T-cell: 5 nTPM
  • naive CD4 T-cell: 5 nTPM
  • memory B-cell: 4.6 nTPM

Brain region

  • cerebellum: 19 nTPM
  • cerebral cortex: 17 nTPM
  • hypothalamus: 16 nTPM
  • basal ganglia: 16 nTPM
  • hippocampal formation: 14 nTPM
  • amygdala: 14 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.48
gnomAD pLI
0.73
DepMap mean gene effect
-0.37
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF9 as an antibody target. Whether an autoantibody or antibody against MARCHF9 could matter depends on whether native MARCHF9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF9 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MARCHF9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MARCHF9. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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