Seroatlas · Human Serome Atlas

MARCHF10

Probable E3 ubiquitin-protein ligase MARCHF10

Also known as: FLJ35757, MARCH-X, MARCH10, MARHA_HUMAN, RNF190

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NA82
Gene: MARCHF10
Ensembl: ENSG00000173838
Chromosome: 17
Canonical length: 808 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Plasma membrane,Cytosol

OverviewNCBI Gene

MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

Canonical amino-acid sequenceUniProt

808 residues, UniProt reviewed canonical sequence.

>Q8NA82|MARCHF10
     1  MLHDARDRQK FFSDVQYLRD MQHKVDSEYQ ACLRRQEYRR DPNEKKRDQF WGQETSFERS
    61  RFSSRSSSKQ SSSEEDALTE PRSSIKISAF KCDSKLPAID QTSVKQKHKS TMTVRKAEKV
   121  DPSEPSPADQ APMVLLRKRK PNLRRFTVSP ESHSPRASGD RSRQKQQWPA KVPVPRGADQ
   181  VVQQEGLMCN TKLKRPNQER RNLVPSSQPM TENAPDRAKK GDPSAPSQSE LHPALSQAFQ
   241  GKNSPQVLSE FSGPPLTPTT VGGPRKASFR FRDEDFYSIL SLNSRRESDD TEEETQSEEC
   301  LWVGVRSPCS PSHHKRSRFG GTSTPQAKNK NFEENAENCR GHSSRRSEPS HGSLRISNAM
   361  EPATERPSAG QRLSQDPGLP DRESATEKDR GGSENAKKSP LSWDTKSEPR QEVGVNAENV
   421  WSDCISVEHR PGTHDSEGYW KDYLNSSQNS LDYFISGRPI SPRSSVNSSY NPPASFMHSA
   481  LRDDIPVDLS MSSTSVHSSD SEGNSGFHVC QPLSPIRNRT PFASAENHNY FPVNSAHEFA
   541  VREAEDTTLT SQPQGAPLYT DLLLNPQGNL SLVDSSSSSP SRMNSEGHLH VSGSLQENTP
   601  FTFFAVSHFP NQNDNGSRMA ASGFTDEKET SKIKADPEKL KKLQESLLEE DSEEEGDLCR
   661  ICQIAGGSPS NPLLEPCGCV GSLQFVHQEC LKKWLKVKIT SGADLGAVKT CEMCKQGLLV
   721  DLGDFNMIEF YQKHQQSQAQ NELMNSGLYL VLLLHLYEQR FAELMRLNHN QVERERLSRN
   781  YPQPRTEENE NSELGDGNEG SISQSQVV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF10 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.66
Highest tissue expression: 130 nTPM

Expression across tissuesHPA

Tissue

testis: 130 nTPM
choroid plexus: 17 nTPM
pituitary gland: 12 nTPM
fallopian tube: 12 nTPM
epididymis: 7 nTPM
basal ganglia: 3.9 nTPM

Single-cell type

late spermatids: 1,992 nCPM
early spermatids: 618 nCPM
late primary spermatocytes: 357 nCPM
respiratory ciliated cells: 246 nCPM
ependymal cells: 212 nCPM
fallopian tube ciliated cells: 142 nCPM

Immune cell

gdT-cell: 0.9 nTPM
naive CD8 T-cell: 0.4 nTPM
MAIT T-cell: 0.3 nTPM
myeloid DC: 0.2 nTPM
T-reg: 0.2 nTPM
memory CD4 T-cell: 0.1 nTPM

Brain region

choroid plexus: 23 nTPM
midbrain: 11 nTPM
spinal cord: 8.2 nTPM
medulla oblongata: 8.1 nTPM
pons: 4.7 nTPM
white matter: 4.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.39
gnomAD pLI: 0
DepMap mean gene effect: -0.12
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein ubiquitination

Molecular functions

Protein domainsUniProt · Pfam · InterPro

Zinc finger, RING-CH-type
Zinc finger, RING/FYVE/PHD-type
RING-CH-type E3 ubiquitin-protein ligase
RING-variant domain
MARCH10, RING-CH finger, C4HC3-type

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF10 as an antibody target. Whether an autoantibody or antibody against MARCHF10 could matter depends on whether native MARCHF10 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF10 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MARCHF10 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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