Seroatlas · Human Serome Atlas

MARCHF1

E3 ubiquitin-protein ligase MARCHF1

Also known as: FLJ20668, MARCH-I, MARCH1, MARH1_HUMAN, RNF171

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8TCQ1
Gene
MARCHF1
Ensembl
ENSG00000145416
Chromosome
4
Canonical length
289 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location
Endoplasmic reticulum,Golgi apparatus,Vesicles

OverviewNCBI Gene

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).[supplied by OMIM, Mar 2010]

Canonical amino-acid sequenceUniProt

289 residues, UniProt reviewed canonical sequence.

>Q8TCQ1|MARCHF1
     1  MLGWCEAIAR NPHRIPNNTR TPEISGDLAD ASQTSTLNEK SPGRSASRSS NISKASSPTT
    61  GTAPRSQSRL SVCPSTQDIC RICHCEGDEE SPLITPCRCT GTLRFVHQSC LHQWIKSSDT
   121  RCCELCKYDF IMETKLKPLR KWEKLQMTTS ERRKIFCSVT FHVIAITCVV WSLYVLIDRT
   181  AEEIKQGNDN GVLEWPFWTK LVVVAIGFTG GLVFMYVQCK VYVQLWRRLK AYNRVIFVQN
   241  CPDTAKKLEK NFSCNVNTDI KDAVVVPVPQ TGANSLPSAE GGPPEVVSV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
2
Mean surface accessibility (rSASA)
0.52
Highest tissue expression
29 nTPM

Expression across tissuesHPA

Tissue

  • lymph node: 29 nTPM
  • spleen: 24 nTPM
  • tonsil: 24 nTPM
  • retina: 21 nTPM
  • appendix: 20 nTPM
  • cerebral cortex: 12 nTPM

Single-cell type

  • rod photoreceptor cells: 2,793 nCPM
  • cone photoreceptor cells: 2,192 nCPM
  • oligodendrocytes: 1,946 nCPM
  • oligodendrocyte progenitor cells: 1,401 nCPM
  • lactotrophs: 1,336 nCPM
  • b-cells: 789 nCPM

Immune cell

  • non-classical monocyte: 46 nTPM
  • myeloid DC: 44 nTPM
  • memory B-cell: 42 nTPM
  • classical monocyte: 42 nTPM
  • intermediate monocyte: 41 nTPM
  • naive B-cell: 36 nTPM

Brain region

  • white matter: 36 nTPM
  • hypothalamus: 33 nTPM
  • cerebral cortex: 27 nTPM
  • midbrain: 25 nTPM
  • medulla oblongata: 24 nTPM
  • basal ganglia: 24 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.77
gnomAD pLI
0.01
DepMap mean gene effect
-0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MARCHF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF1 as an antibody target. Whether an autoantibody or antibody against MARCHF1 could matter depends on whether native MARCHF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF1 is annotated at the cell surface, where native MARCHF1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label MARCHF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MARCHF1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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