Seroatlas · Human Serome Atlas

LDAF1

Lipid droplet assembly factor 1

Also known as: LDAF1_HUMAN, promethin, TMEM159

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96B96
Gene: LDAF1
Ensembl: ENSG00000011638
Chromosome: 16
Canonical length: 161 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Vesicles

OverviewNCBI Gene

Involved in lipid droplet formation. Located in endoplasmic reticulum membrane and lipid droplet. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

161 residues, UniProt reviewed canonical sequence.

>Q96B96|LDAF1
     1  MAKEEPQSIS RDLQELQKKL SLLIDSFQNN SKVVAFMKSP VGQYLDSHPF LAFTLLVFIV
    61  MSAVPVGFFL LIVVLTTLAA LLGVIILEGL VISVGGFSLL CILCGLGFVS LAMSGMMIAS
   121  YVVVSSLISC WFSPRPLTQQ NTSCDFLPAM KSAEFEGLYQ E

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LDAF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 4
Mean surface accessibility (rSASA): 0.53
Highest tissue expression: 120 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 120 nTPM
tongue: 93 nTPM
skin: 86 nTPM
heart muscle: 83 nTPM
blood vessel: 58 nTPM
esophagus: 56 nTPM

Single-cell type

cardiomyocytes: 72 nCPM
esophageal apical cells: 52 nCPM
urothelial cells: 37 nCPM
papillary tip epithelial cells: 31 nCPM
distal convoluted tubule cells: 31 nCPM
ocular epithelial cells: 28 nCPM

Immune cell

basophil: 20 nTPM
T-reg: 15 nTPM
plasmacytoid DC: 14 nTPM
memory CD4 T-cell: 13 nTPM
memory B-cell: 12 nTPM
non-classical monocyte: 10 nTPM

Brain region

basal ganglia: 15 nTPM
cerebellum: 11 nTPM
hypothalamus: 9.7 nTPM
cerebral cortex: 6.7 nTPM
pons: 6.6 nTPM
midbrain: 5.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.64
gnomAD pLI: 0
DepMap mean gene effect: 0
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

lipid droplet formation

Cellular components

Protein domainsUniProt · Pfam · InterPro

Lipid droplet assembly factor 1
Promethin

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of LDAF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

BSCL2

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads LDAF1 as an antibody target. Whether an autoantibody or antibody against LDAF1 could matter depends on whether native LDAF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

LDAF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label LDAF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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