Seroatlas · Human Serome Atlas

ISM2

Isthmin-2

Also known as: FLJ32147, ISM2_HUMAN, TAIL1, THSD3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6H9L7
Gene: ISM2
Ensembl: ENSG00000100593
Chromosome: 14
Canonical length: 571 aa
Protein class: Predicted secreted proteins
Subcellular location: Nucleoplasm,Centrosome,Cytosol
Secretome location: Secreted in female reproductive system

OverviewNCBI Gene

The protein encoded by this gene contains a type 1 thrombospondin domain, which is present in thrombospondin, a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Mar 2009]

Canonical amino-acid sequenceUniProt

571 residues, UniProt reviewed canonical sequence.

>Q6H9L7|ISM2
     1  MRALRDRAGL LLCVLLLAAL LEAALGLPVK KPRLRGPRPG SLTRLAEVSA SPDPRPLKEE
    61  EEAPLLPRTH LQAEPHQHGC WTVTEPAAMT PGNATPPRTP EVTPLRLELQ KLPGLANTTL
   121  STPNPDTQAS ASPDPRPLRE EEEARLLPRT HLQAELHQHG CWTVTEPAAL TPGNATPPRT
   181  QEVTPLLLEL QKLPELVHAT LSTPNPDNQV TIKVVEDPQA EVSIDLLAEP SNPPPQDTLS
   241  WLPALWSFLW GDYKGEEKDR APGEKGEEKE EDEDYPSEDI EGEDQEDKEE DEEEQALWFN
   301  GTTDNWDQGW LAPGDWVFKD SVSYDYEPQK EWSPWSPCSG NCSTGKQQRT RPCGYGCTAT
   361  ETRTCDLPSC PGTEDKDTLG LPSEEWKLLA RNATDMHDQD VDSCEKWLNC KSDFLIKYLS
   421  QMLRDLPSCP CAYPLEAMDS PVSLQDEHQG RSFRWRDASG PRERLDIYQP TARFCLRSML
   481  SGESSTLAAQ HCCYDEDSRL LTRGKGAGMP NLISTDFSPK LHFKFDTTPW ILCKGDWSRL
   541  HAVLPPNNGR ACTDNPLEEE YLAQLQEAKE Y

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ISM2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.52
Highest tissue expression: 201 nTPM

Expression across tissuesHPA

Tissue

placenta: 201 nTPM
epididymis: 3.4 nTPM
basal ganglia: 2.2 nTPM
prostate: 1.8 nTPM
testis: 1.6 nTPM
choroid plexus: 1.5 nTPM

Single-cell type

extravillous trophoblasts: 1,123 nCPM
migrating cytotrophoblasts: 494 nCPM
cytotrophoblasts: 334 nCPM
syncytiotrophoblasts: 193 nCPM
oocytes: 9.2 nCPM
epicardial cells: 9.1 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

basal ganglia: 2.6 nTPM
choroid plexus: 1.9 nTPM
hypothalamus: 1.8 nTPM
amygdala: 0.8 nTPM
cerebral cortex: 0.8 nTPM
midbrain: 0.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.18
gnomAD pLI: 0
gnomAD missense Z: 0.35
DepMap mean gene effect: -0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Cellular components

extracellular region

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ISM2 as an antibody target. Whether an autoantibody or antibody against ISM2 could matter depends on whether native ISM2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ISM2 is annotated as secreted, so native ISM2 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label ISM2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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