IL33

Protein identityUniProt · HPA

UniProt accession

O95760

Gene

IL33

Ensembl

ENSG00000137033

Chromosome

9

Canonical length

270 aa

Protein class

Predicted intracellular proteins, Predicted secreted proteins

Subcellular location

Nucleoplasm,Vesicles

Secretome location

Secreted to blood

OverviewNCBI Gene

The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Canonical amino-acid sequenceUniProt

270 residues, UniProt reviewed canonical sequence.

>O95760|IL33
     1  MKPKMKYSTN KISTAKWKNT ASKALCFKLG KSQQKAKEVC PMYFMKLRSG LMIKKEACYF
    61  RRETTKRPSL KTGRKHKRHL VLAACQQQST VECFAFGISG VQKYTRALHD SSITGISPIT
   121  EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK
   181  MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI
   241  GVKDNHLALI KVDSSENLCT ENILFKLSET

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against IL33 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.44

Highest tissue expression

114 nTPM

Expression across tissuesHPA

ReferencesPubMed · IEDB

Publications for IL33 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.75

gnomAD pLI

0

gnomAD missense Z

-1.03

DepMap mean gene effect

0.13

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• antibacterial innate immune response
• defense response to virus
• extrinsic apoptotic signaling pathway
• gene expression
• interleukin-33-mediated signaling pathway
• macrophage differentiation
• microglial cell activation involved in immune response
• microglial cell proliferation
• negative regulation of immunoglobulin production
• negative regulation of inflammatory response to wounding
• negative regulation of leukocyte migration
• negative regulation of T-helper 1 type immune response
• negative regulation of transcription by RNA polymerase II
• negative regulation of type II interferon production
• positive regulation of chemokine production
• positive regulation of cytokine production
• positive regulation of gene expression
• positive regulation of glycoprotein biosynthetic process
• positive regulation of immunoglobulin production
• positive regulation of inflammatory response
• positive regulation of interleukin-13 production
• positive regulation of interleukin-4 production
• positive regulation of interleukin-5 production
• positive regulation of interleukin-6 production
• positive regulation of macrophage activation
• positive regulation of MHC class I biosynthetic process
• positive regulation of MHC class II biosynthetic process
• positive regulation of neuroinflammatory response
• positive regulation of proteasomal ubiquitin-dependent protein catabolic process
• positive regulation of transcription by RNA polymerase II
• positive regulation of tumor necrosis factor production
• positive regulation of type 2 immune response
• protein import into nucleus
• response to wounding
• type 2 immune response
• negative regulation of macrophage proliferation
• positive regulation of cellular defense response

Molecular functions

• cytokine activity
• interleukin-33 receptor binding

Cellular components

• chromosome
• cytoplasm
• extracellular region
• extracellular space
• nucleoplasm
• nucleus
• transport vesicle

Protein domainsUniProt · Pfam · InterPro

• Interleukin-33
• Interleukin 33, C-terminal
• Interleukin 33

KeywordsUniProt

• Chromosome
• Cytokine
• Cytoplasm
• Cytoplasmic vesicle
• Nucleus
• Secreted
• Transcription