HLF
Hepatic leukemia factor
Also known as: HLF_HUMAN, MGC33822
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q16534
- Gene
- HLF
- Ensembl
- ENSG00000108924
- Chromosome
- 17
- Canonical length
- 295 aa
- Protein class
- Cancer-related genes, Disease related genes, Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm
- Quaternary structure
- Homodimer
OverviewNCBI Gene
This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
295 residues, UniProt reviewed canonical sequence.
>Q16534|HLF
1 MEKMSRPLPL NPTFIPPPYG VLRSLLENPL KLPLHHEDAF SKDKDKEKKL DDESNSPTVP
61 QSAFLGPTLW DKTLPYDGDT FQLEYMDLEE FLSENGIPPS PSQHDHSPHP PGLQPASSAA
121 PSVMDLSSRA SAPLHPGIPS PNCMQSPIRP GQLLPANRNT PSPIDPDTIQ VPVGYEPDPA
181 DLALSSIPGQ EMFDPRKRKF SEEELKPQPM IKKARKVFIP DDLKDDKYWA RRRKNNMAAK
241 RSRDARRLKE NQIAIRASFL EKENSALRQE VADLRKELGK CKNILAKYEA RHGPLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against HLF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.58
- Highest tissue expression
- 114 nTPM
Expression across tissuesHPA
Tissue
- liver: 114 nTPM
- retina: 40 nTPM
- cerebral cortex: 38 nTPM
- ovary: 35 nTPM
- thyroid gland: 32 nTPM
- esophagus: 25 nTPM
Single-cell type
- hepatocytes: 264 nCPM
- retinal amacrine cells: 206 nCPM
- retinal bipolar cells: 196 nCPM
- distal convoluted tubule cells: 125 nCPM
- hematopoietic stem cells: 120 nCPM
- renal connecting tubule cells: 112 nCPM
Immune cell
- memory CD4 T-cell: 0.5 nTPM
- T-reg: 0.4 nTPM
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
Brain region
- cerebral cortex: 205 nTPM
- basal ganglia: 132 nTPM
- white matter: 129 nTPM
- thalamus: 88 nTPM
- hippocampal formation: 82 nTPM
- midbrain: 71 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.31
- gnomAD pLI
- 0.95
- gnomAD missense Z
- 2.28
- DepMap mean gene effect
- -0.1
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- positive regulation of transcription by RNA polymerase II
- regulation of transcription by RNA polymerase II
- rhythmic process
- skeletal muscle cell differentiation
Molecular functions
- DNA binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity, RNA polymerase II-specific
- double-stranded DNA binding
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads HLF as an antibody target. Whether an autoantibody or antibody against HLF could matter depends on whether native HLF is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
HLF is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label HLF as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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