HEMK2

Protein identityUniProt · HPA

UniProt accession

Q9Y5N5

Gene

HEMK2

Ensembl

ENSG00000156239

Chromosome

21

Canonical length

214 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Centrosome

OverviewNCBI Gene

This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Mar 2023]

Canonical amino-acid sequenceUniProt

214 residues, UniProt reviewed canonical sequence.

>Q9Y5N5|HEMK2
     1  MAGENFATPF HGHVGRGAFS DVYEPAEDTF LLLDALEAAA AELAGVEICL EVGSGSGVVS
    61  AFLASMIGPQ ALYMCTDINP EAAACTLETA RCNKVHIQPV ITDLVKGLLP RLTEKVDLLV
   121  FNPPYVVTPP QEVGSHGIEA AWAGGRNGRE VMDRFFPLVP DLLSPRGLFY LVTIKENNPE
   181  EILKIMKTKG LQGTTALSRQ AGQETLSVLK FTKS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HEMK2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

7.8 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.68

gnomAD pLI

0

DepMap mean gene effect

-0.42

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• arsonoacetate metabolic process
• methylation
• negative regulation of gene expression, epigenetic
• peptidyl-glutamine methylation
• positive regulation of cell growth
• toxin metabolic process
• transcription initiation-coupled chromatin remodeling

Molecular functions

• arsenite methyltransferase activity
• nucleic acid binding
• protein methyltransferase activity
• protein-glutamine N-methyltransferase activity
• S-adenosyl-L-methionine binding
• S-adenosylmethionine-dependent methyltransferase activity
• site-specific DNA-methyltransferase (adenine-specific) activity
• histone H4K12 methyltransferase activity

Cellular components

• cytosol
• nucleus
• protein-containing complex
• eRF1 methyltransferase complex

Protein domainsUniProt · Pfam · InterPro

• DNA methylase, N-6 adenine-specific, conserved site
• Methyltransferase small domain
• S-adenosyl-L-methionine-dependent methyltransferase superfamily
• Methyltransferase small domain
• Eukaryotic/archaeal PrmC-related
• Release factor glutamine methyltransferase Mtq2-like

KeywordsUniProt

• Chromatin regulator
• Methyltransferase
• Nucleus
• S-adenosyl-L-methionine
• Transferase
• Ubl conjugation