GUCY1B1

Protein identityUniProt · HPA

UniProt accession

Q02153

Gene

GUCY1B1

Ensembl

ENSG00000061918

Chromosome

4

Canonical length

619 aa

Protein class

Enzymes, FDA approved drug targets, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Canonical amino-acid sequenceUniProt

619 residues, UniProt reviewed canonical sequence.

>Q02153|GUCY1B1
     1  MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD LVAAASKVLN
    61  LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN LDALHDHLAT IYPGMRAPSF
   121  RCTDAEKGKG LILHYYSERE GLQDIVIGII KTVAQQIHGT EIDMKVIQQR NEECDHTQFL
   181  IEEKESKEED FYEDLDRFEE NGTQESRISP YTFCKAFPFH IIFDRDLVVT QCGNAIYRVL
   241  PQLQPGNCSL LSVFSLVRPH IDISFHGILS HINTVFVLRS KEGLLDVEKL ECEDELTGTE
   301  ISCLRLKGQM IYLPEADSIL FLCSPSVMNL DDLTRRGLYL SDIPLHDATR DLVLLGEQFR
   361  EEYKLTQELE ILTDRLQLTL RALEDEKKKT DTLLYSVLPP SVANELRHKR PVPAKRYDNV
   421  TILFSGIVGF NAFCSKHASG EGAMKIVNLL NDLYTRFDTL TDSRKNPFVY KVETVGDKYM
   481  TVSGLPEPCI HHARSICHLA LDMMEIAGQV QVDGESVQIT IGIHTGEVVT GVIGQRMPRY
   541  CLFGNTVNLT SRTETTGEKG KINVSEYTYR CLMSPENSDP QFHLEHRGPV SMKGKKEPMQ
   601  VWFLSRKNTG TEETKQDDD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GUCY1B1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

47 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.68

gnomAD pLI

0

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• blood circulation
• cellular response to nitric oxide
• cGMP biosynthetic process
• cGMP-mediated signaling
• nitric oxide mediated signal transduction
• nitric oxide-cGMP-mediated signaling
• response to oxygen levels

Molecular functions

• adenylate cyclase activity
• GTP binding
• guanylate cyclase activity
• heme binding
• Hsp90 protein binding
• metal ion binding
• nitric oxide binding
• protein-containing complex binding
• signaling receptor activity
• cytidylate cyclase activity

Cellular components

• cytosol
• glutamatergic synapse
• guanylate cyclase complex, soluble
• presynaptic active zone cytoplasmic component

Protein domainsUniProt · Pfam · InterPro

• Adenylyl cyclase class-3/4/guanylyl cyclase
• Heme NO-binding
• Haem NO binding associated
• Adenylyl cyclase class-4/guanylyl cyclase, conserved site
• NO signalling/Golgi transport ligand-binding domain superfamily
• Nucleotide cyclase
• H-NOX domain superfamily
• Haem NO binding associated domain superfamily
• Adenylate and Guanylate cyclase catalytic domain
• Haem-NO-binding
• Heme NO binding associated

KeywordsUniProt

• cGMP biosynthesis
• Cytoplasm
• GTP-binding
• Heme
• Iron
• Lyase
• Metal-binding
• Nucleotide-binding