GUCY1A2

Protein identityUniProt · HPA

UniProt accession

P33402

Gene

GUCY1A2

Ensembl

ENSG00000152402

Chromosome

11

Canonical length

732 aa

Protein class

Enzymes, FDA approved drug targets, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Canonical amino-acid sequenceUniProt

732 residues, UniProt reviewed canonical sequence.

>P33402|GUCY1A2
     1  MSRRKISSES FSSLGSDYLE TSPEEEGECP LSRLCWNGSR SPPGPLEPSP AAAAAAAAPA
    61  PTPAASAAAA AATAGARRVQ RRRRVNLDSL GESISRLTAP SPQTIQQTLK RTLQYYEHQV
   121  IGYRDAEKNF HNISNRCSYA DHSNKEEIED VSGILQCTAN ILGLKFEEIQ KRFGEEFFNI
   181  CFHENERVLR AVGGTLQDFF NGFDALLEHI RTSFGKQATL ESPSFLCKEL PEGTLMLHYF
   241  HPHHIVGFAM LGMIKAAGKK IYRLDVEVEQ VANEKLCSDV SNPGNCSCLT FLIKECENTN
   301  IMKNLPQGTS QVPADLRISI NTFCRAFPFH LMFDPSMSVL QLGEGLRKQL RCDTHKVLKF
   361  EDCFEIVSPK VNATFERVLL RLSTPFVIRT KPEASGSENK DKVMEVKGQM IHVPESNSIL
   421  FLGSPCVDKL DELMGRGLHL SDIPIHDATR DVILVGEQAK AQDGLKKRMD KLKATLERTH
   481  QALEEEKKKT VDLLYSIFPG DVAQQLWQGQ QVQARKFDDV TMLFSDIVGF TAICAQCTPM
   541  QVISMLNELY TRFDHQCGFL DIYKVETIGD AYCVAAGLHR KSLCHAKPIA LMALKMMELS
   601  EEVLTPDGRP IQMRIGIHSG SVLAGVVGVR MPRYCLFGNN VTLASKFESG SHPRRINVSP
   661  TTYQLLKREE SFTFIPRSRE ELPDNFPKEI PGICYFLEVR TGPKPPKPSL SSSRIKKVSY
   721  NIGTMFLRET SL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GUCY1A2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.38

Highest tissue expression

17 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.43

gnomAD pLI

0.31

gnomAD missense Z

2.36

DepMap mean gene effect

0.13

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cGMP-mediated signaling
• nitric oxide mediated signal transduction
• positive regulation of nitric oxide mediated signal transduction
• response to oxygen levels
• signal transduction

Molecular functions

• GTP binding
• guanylate cyclase activity
• heme binding

Cellular components

• cytosol
• guanylate cyclase complex, soluble

Protein domainsUniProt · Pfam · InterPro

• Adenylyl cyclase class-3/4/guanylyl cyclase
• Heme NO-binding
• Haem NO binding associated
• Adenylyl cyclase class-4/guanylyl cyclase, conserved site
• NO signalling/Golgi transport ligand-binding domain superfamily
• Nucleotide cyclase
• H-NOX domain superfamily
• Haem NO binding associated domain superfamily
• Adenylate and Guanylate cyclase catalytic domain
• Haem-NO-binding
• Heme NO binding associated

KeywordsUniProt

• cGMP biosynthesis
• Cytoplasm
• GTP-binding
• Lyase
• Nucleotide-binding