Seroatlas · Human Serome Atlas

GRAMD4

GRAM domain-containing protein 4

Also known as: DIP, GRAM4_HUMAN, KIAA0767

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6IC98
Gene: GRAMD4
Ensembl: ENSG00000075240
Chromosome: 22
Canonical length: 578 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

GRAMD4 is a mitochondrial effector of E2F1 (MIM 189971)-induced apoptosis (Stanelle et al., 2005 [PubMed 15565177]).[supplied by OMIM, Jan 2011]

Canonical amino-acid sequenceUniProt

578 residues, UniProt reviewed canonical sequence.

>Q6IC98|GRAMD4
     1  MLRRLDKIRF RGHKRDDFLD LAESPNASDT ECSDEIPLKV PRTSPRDSEE LRDPAGPGTL
    61  IMATGVQDFN RTEFDRLNEI KGHLEIALLE KHFLQEELRK LREETNAEML RQELDRERQR
   121  RMELEQKVQE VLKARTEEQM AQQPPKGQAQ ASNGAERRSQ GLSSRLQKWF YERFGEYVED
   181  FRFQPEENTV ETEEPLSARR LTENMRRLKR GAKPVTNFVK NLSALSDWYS VYTSAIAFTV
   241  YMNAVWHGWA IPLFLFLAIL RLSLNYLIAR GWRIQWSIVP EVSEPVEPPK EDLTVSEKFQ
   301  LVLDVAQKAQ NLFGKMADIL EKIKNLFMWV QPEITQKLYV ALWAAFLASC FFPYRLVGLA
   361  VGLYAGIKFF LIDFIFKRCP RLRAKYDTPY IIWRSLPTDP QLKERSSAAV SRRLQTTSSR
   421  SYVPSAPAGL GKEEDAGRFH STKKGNFHEI FNLTENERPL AVCENGWRCC LINRDRKMPT
   481  DYIRNGVLYV TENYLCFESS KSGSSKRNKV IKLVDITDIQ KYKVLSVLPG SGMGIAVSTP
   541  STQKPLVFGA MVHRDEAFET ILSQYIKITS AAASGGDS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GRAMD4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 3
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 167 nTPM

Expression across tissuesHPA

Tissue

liver: 167 nTPM
adrenal gland: 55 nTPM
skin: 43 nTPM
heart muscle: 40 nTPM
colon: 38 nTPM
small intestine: 34 nTPM

Single-cell type

thymocytes: 132 nCPM
pancreatic islet cells: 127 nCPM
megakaryocyte-erythroid progenitors: 121 nCPM
enterocytes: 120 nCPM
retinal pigment epithelial cells: 81 nCPM
colonocytes: 76 nCPM

Immune cell

NK-cell: 3.9 nTPM
plasmacytoid DC: 2.4 nTPM
myeloid DC: 2 nTPM
T-reg: 1.9 nTPM
classical monocyte: 1.4 nTPM
basophil: 1.2 nTPM

Brain region

cerebral cortex: 62 nTPM
basal ganglia: 54 nTPM
hippocampal formation: 53 nTPM
amygdala: 52 nTPM
white matter: 50 nTPM
pons: 47 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.16
gnomAD pLI: 1
gnomAD missense Z: 2.56
DepMap mean gene effect: 0.11
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

GRAM domain
PH-like domain superfamily
GRAM domain
GRAMDC4, PH-GRAM domain
GRAM domain-containing protein 4

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of GRAMD4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

RTN4

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GRAMD4 as an antibody target. Whether an autoantibody or antibody against GRAMD4 could matter depends on whether native GRAMD4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GRAMD4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GRAMD4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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