GPR65
G-protein coupled receptor 65
Also known as: GPR65_HUMAN, hTDAG8, TDAG8
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q8IYL9
- Gene
- GPR65
- Ensembl
- ENSG00000140030
- Chromosome
- 14
- Canonical length
- 337 aa
- Protein class
- G-protein coupled receptors, Predicted membrane proteins
- Subcellular location
- Nucleoli,Vesicles,Cytosol
OverviewNCBI Gene
Enables G protein-coupled receptor activity. Involved in several processes, including activation of GTPase activity; positive regulation of stress fiber assembly; and response to acidic pH. Located in plasma membrane. [provided by Alliance of Genome Resources, Apr 2025]
Canonical amino-acid sequenceUniProt
337 residues, UniProt reviewed canonical sequence.
>Q8IYL9|GPR65
1 MNSTCIEEQH DLDHYLFPIV YIFVIIVSIP ANIGSLCVSF LQAKKESELG IYLFSLSLSD
61 LLYALTLPLW IDYTWNKDNW TFSPALCKGS AFLMYMNFYS STAFLTCIAV DRYLAVVYPL
121 KFFFLRTRRF ALMVSLSIWI LETIFNAVML WEDETVVEYC DAEKSNFTLC YDKYPLEKWQ
181 INLNLFRTCT GYAIPLVTIL ICNRKVYQAV RHNKATENKE KKRIIKLLVS ITVTFVLCFT
241 PFHVMLLIRC ILEHAVNFED HSNSGKRTYT MYRITVALTS LNCVADPILY CFVTETGRYD
301 MWNILKFCTG RCNTSQRQRK RILSVSTKDT MELEVLELocalizationUniProt · AlphaFold · HPA
Whether an antibody against GPR65 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 7
- Mean surface accessibility (rSASA)
- 0.28
- Highest tissue expression
- 12 nTPM
Expression across tissuesHPA
Tissue
- bone marrow: 12 nTPM
- spleen: 9.1 nTPM
- appendix: 8.6 nTPM
- lymph node: 8.2 nTPM
- tonsil: 6.2 nTPM
- lung: 4.1 nTPM
Single-cell type
- mast cells: 547 nCPM
- neutrophils: 241 nCPM
- nk-cells: 217 nCPM
- kupffer cells: 168 nCPM
- t-cells: 143 nCPM
- innate lymphoid cells: 135 nCPM
Immune cell
- MAIT T-cell: 63 nTPM
- NK-cell: 49 nTPM
- eosinophil: 44 nTPM
- basophil: 39 nTPM
- gdT-cell: 34 nTPM
- non-classical monocyte: 32 nTPM
Brain region
- white matter: 5 nTPM
- medulla oblongata: 4.3 nTPM
- thalamus: 3.2 nTPM
- pons: 2.8 nTPM
- hypothalamus: 2.6 nTPM
- spinal cord: 2.6 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.83
- gnomAD pLI
- 0.11
- gnomAD missense Z
- 0.7
- DepMap mean gene effect
- 0.1
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- adenylate cyclase-activating G protein-coupled receptor signaling pathway
- apoptotic process
- cellular response to acidic pH
- G protein-coupled receptor signaling pathway
- immune response
- positive regulation of stress fiber assembly
- positive regulation of T-helper cell differentiation
- response to acidic pH
- positive regulation of interleukin-22 production
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads GPR65 as an antibody target. Whether an autoantibody or antibody against GPR65 could matter depends on whether native GPR65 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
GPR65 is annotated at the cell surface, where native GPR65 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label GPR65 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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