Seroatlas · Human Serome Atlas

GPR25

Probable G-protein coupled receptor 25

Also known as: GPR25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O00155
Gene: GPR25
Ensembl: ENSG00000170128
Chromosome: 1
Canonical length: 361 aa
Protein class: G-protein coupled receptors, Predicted membrane proteins

OverviewNCBI Gene

This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

361 residues, UniProt reviewed canonical sequence.

>O00155|GPR25
     1  MAPTEPWSPS PGSAPWDYSG LDGLEELELC PAGDLPYGYV YIPALYLAAF AVGLLGNAFV
    61  VWLLAGRRGP RRLVDTFVLH LAAADLGFVL TLPLWAAAAA LGGRWPFGDG LCKLSSFALA
   121  GTRCAGALLL AGMSVDRYLA VVKLLEARPL RTPRCALASC CGVWAVALLA GLPSLVYRGL
   181  QPLPGGQDSQ CGEEPSHAFQ GLSLLLLLLT FVLPLVVTLF CYCRISRRLR RPPHVGRARR
   241  NSLRIIFAIE STFVGSWLPF SALRAVFHLA RLGALPLPCP LLLALRWGLT IATCLAFVNS
   301  CANPLIYLLL DRSFRARALD GACGRTGRLA RRISSASSLS RDDSSVFRCR AQAANTASAS
   361  W

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GPR25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 7
Mean surface accessibility (rSASA): 0.35
Highest tissue expression: 1.8 nTPM

Expression across tissuesHPA

Tissue

stomach: 1.8 nTPM
appendix: 0.4 nTPM
lung: 0.4 nTPM
small intestine: 0.4 nTPM
spleen: 0.4 nTPM
testis: 0.4 nTPM

Single-cell type

late primary spermatocytes: 0.4 nCPM
innate lymphoid cells: 0.3 nCPM
late spermatids: 0.3 nCPM
nk-cells: 0.2 nCPM
plasma cells: 0.2 nCPM
t-cells: 0.2 nCPM

Immune cell

T-reg: 44 nTPM
memory CD4 T-cell: 28 nTPM
memory B-cell: 15 nTPM
MAIT T-cell: 11 nTPM
gdT-cell: 11 nTPM
memory CD8 T-cell: 8.6 nTPM

Brain region

hypothalamus: 0.8 nTPM
basal ganglia: 0.6 nTPM
amygdala: 0.5 nTPM
cerebral cortex: 0.5 nTPM
hippocampal formation: 0.4 nTPM
midbrain: 0.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.26
gnomAD pLI: 0.01
gnomAD missense Z: 1.68
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

plasma membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GPR25 as an antibody target. Whether an autoantibody or antibody against GPR25 could matter depends on whether native GPR25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GPR25 is annotated at the cell surface, where native GPR25 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label GPR25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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