GPR149

Protein identityUniProt · HPA

UniProt accession

Q86SP6

Gene

GPR149

Ensembl

ENSG00000174948

Chromosome

3

Canonical length

731 aa

Protein class

G-protein coupled receptors, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes a seven-transmembrane G protein coupled receptor (GPCR) class A family member. Although categorized as a class A GPCR, the encoded protein lacks the first two charged amino acids of the highly conserved Asp-Arg-Tyr (DRY) motif found in the third transmembrane helix of class A receptors which is important for efficient G protein-coupled signal transduction. Mice with a knockout of the orthologous gene are viable and have normal maturation of the ovarian follicle, but show enhanced fertility and ovulation. All GPCRs have a common structural architecture consisting of seven transmembrane alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptor, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes. [provided by RefSeq, Jul 2017]

Canonical amino-acid sequenceUniProt

731 residues, UniProt reviewed canonical sequence.

>Q86SP6|GPR149
     1  MSLFLSNLST NDSSLWKENH NSTDLLNPPG TLNIYLFCLT CLMTFAALVG SIYSLISLLK
    61  MQNRTVVSML VASWSVDDLM SVLSVTIFMF LQWPNEVPGY FQFLCTTSAL MYLCQGLSSN
   121  LKATLLVSYN FYTMHRGVGS QTASRRSGQV LGVVLTVWAA SLLLSALPLC GWGAFVRTPW
   181  GCLVDCSSSY VLFLSIVYAL AFGLLVGLSV PLTHRLLCSE EPPRLHSNYQ EISRGASIPG
   241  TPPTAGRVVS LSPEDAPGPS LRRSGGCSPS SDTVFGPGAP AAAGAEACRR ENRGTLYGTR
   301  SFTVSVAQKR FALILALTKV VLWLPMMMHM VVQNVVGFQS LPLETFSFLL TLLATTVTPV
   361  FVLSKRWTHL PCGCIINCRQ NAYAVASDGK KIKRKGFEFN LSFQKSYGIY KIAHEDYYDD
   421  DENSIFYHNL MNSECETTKD PQRDNRNIFN AIKVEISTTP SLDSSTQRGI NKCTNTDITE
   481  AKQDSNNKKD AFSDKTGGDI NYEETTFSEG PERRLSHEES QKPDLSDWEW CRSKSERTPR
   541  QRSGYALAIP LCAFQGTVSL HAPTGKTLSL STYEVSAEGQ KITPASKKIE VYRSKSVGHE
   601  PNSEDSSSTF VDTSVKIHLE VLEICDNEEA LDTVSIISNI SQSSTQVRSP SLRYSRKENR
   661  FVSCDLGETA SYSLFLPTSN PDGDINISIP DTVEAHRQNS KRQHQERDGY QEEIQLLNKA
   721  YRKREEESKG S

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GPR149 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

7

Mean surface accessibility (rSASA)

0.49

Highest tissue expression

2.4 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.1

gnomAD pLI

0

gnomAD missense Z

-0.93

DepMap mean gene effect

-0.09

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• antral ovarian follicle growth
• neuropeptide signaling pathway
• preantral ovarian follicle growth
• negative regulation of ovulation

Molecular functions

• G protein-coupled receptor activity
• neuropeptide binding

Cellular components

• neuron projection
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• GPCR, rhodopsin-like, 7TM

KeywordsUniProt

• Cell membrane
• Disulfide bond
• G-protein coupled receptor
• Glycoprotein
• Membrane
• Receptor
• Transducer
• Transmembrane
• Transmembrane helix