Seroatlas · Human Serome Atlas

GNLY

Granulysin

Also known as: D2S69E, GNLY_HUMAN, LAG-2, LAG2, NKG5, TLA519

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P22749
Gene: GNLY
Ensembl: ENSG00000115523
Chromosome: 2
Canonical length: 145 aa
Protein class: Predicted intracellular proteins, Predicted secreted proteins, Transporters
Secretome location: Secreted to blood

OverviewNCBI Gene

The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

145 residues, UniProt reviewed canonical sequence.

>P22749|GNLY
     1  MATWALLLLA AMLLGNPGLV FSRLSPEYYD LARAHLRDEE KSCPCLAQEG PQGDLLTKTQ
    61  ELGRDYRTCL TIVQKLKKMV DKPTQRSVSN AATRVCRTGR SRWRDVCRNF MRRYQSRVTQ
   121  GLVAGETAQQ ICEDLRLCIP STGPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GNLY can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.47
Highest tissue expression: 272 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 272 nTPM
spleen: 81 nTPM
endometrium: 68 nTPM
lung: 35 nTPM
lymph node: 21 nTPM
tonsil: 17 nTPM

Single-cell type

nk-cells: 3,025 nCPM
t-cells: 477 nCPM
innate lymphoid cells: 438 nCPM
endometrial secretory cells: 43 nCPM
platelets: 38 nCPM
microglia: 29 nCPM

Immune cell

NK-cell: 17,662 nTPM
total PBMC: 8,133 nTPM
gdT-cell: 7,897 nTPM
memory CD8 T-cell: 3,346 nTPM
naive CD8 T-cell: 2,936 nTPM
MAIT T-cell: 2,152 nTPM

Brain region

medulla oblongata: 12 nTPM
pons: 11 nTPM
thalamus: 7.1 nTPM
hypothalamus: 7 nTPM
white matter: 6.5 nTPM
midbrain: 5.7 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.86
gnomAD pLI: 0
gnomAD missense Z: 0.14
DepMap mean gene effect: -0.12
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GNLY as an antibody target. Whether an autoantibody or antibody against GNLY could matter depends on whether native GNLY is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GNLY is annotated as secreted, so native GNLY circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label GNLY as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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