Seroatlas · Human Serome Atlas

GALNT8

Probable polypeptide N-acetylgalactosaminyltransferase 8

Also known as: GALNAC-T8, GALT8_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9NY28
Gene: GALNT8
Ensembl: ENSG00000130035
Chromosome: 12
Canonical length: 637 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Golgi apparatus,Vesicles

OverviewNCBI Gene

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

637 residues, UniProt reviewed canonical sequence.

>Q9NY28|GALNT8
     1  MMFWRKLPKA LFIGLTLAIA VNLLLVFSSK GTLQNLFTGG LHRELPLHLN KRYGAVIKRL
    61  SHLEVELQDL KESMKLALRQ QENVNSTLKR AKDEVRPLLK AMETKVNETK KHKTQMKLFP
   121  HSQLFRQWGE DLSEAQQKAA QDLFRKFGYN AYLSNQLPLN RTIPDTRDYR CLRKTYPSQL
   181  PSLSVILIFV NEALSIIQRA ITSIINRTPS RLLKEIILVD DFSSNGELKV HLDEKIKLYN
   241  QKYPGLLKII RHPERKGLAQ ARNTGWEAAT ADVVAILDAH IEVNVGWAEP ILARIQEDRT
   301  VIVSPVFDNI RFDTFKLDKY ELAVDGFNWE LWCRYDALPQ AWIDLHDVTA PVKSPSIMGI
   361  LAANRHFLGE IGSLDGGMLI YGGENVELSL RVWQCGGKVE ILPCSRIAHL ERHHKPYALD
   421  LTAALKRNAL RVAEIWMDEH KHMVYLAWNI PLQNSGIDFG DVSSRMALRE KLKCKTFDWY
   481  LKNVYPLLKP LHTIVGYGRM KNLLDENVCL DQGPVPGNTP IMYYCHEFSS QNVYYHLTGE
   541  LYVGQLIAEA SASDRCLTDP GKAEKPTLEP CSKAAKNRLH IYWDFKPGGA VINRDTKRCL
   601  EMKKDLLGSH VLVLQTCSTQ VWEIQHTVRD WGQTNSQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GALNT8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 29 nTPM

Expression across tissuesHPA

Tissue

rectum: 29 nTPM
colon: 13 nTPM
small intestine: 12 nTPM
testis: 10 nTPM
duodenum: 5.3 nTPM
cerebral cortex: 4.1 nTPM

Single-cell type

goblet cells: 223 nCPM
late spermatids: 141 nCPM
ependymal cells: 94 nCPM
early spermatids: 79 nCPM
oligodendrocyte progenitor cells: 46 nCPM
adrenal medulla cells: 32 nCPM

Immune cell

gdT-cell: 0.3 nTPM
NK-cell: 0.2 nTPM
memory CD8 T-cell: 0.1 nTPM
naive B-cell: 0.1 nTPM
plasmacytoid DC: 0.1 nTPM
T-reg: 0.1 nTPM

Brain region

white matter: 4.6 nTPM
cerebral cortex: 3.6 nTPM
medulla oblongata: 3.4 nTPM
pons: 3.2 nTPM
basal ganglia: 3.1 nTPM
thalamus: 3.1 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.39
gnomAD pLI: 0
gnomAD missense Z: -0.02
DepMap mean gene effect: 0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GALNT8 as an antibody target. Whether an autoantibody or antibody against GALNT8 could matter depends on whether native GALNT8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GALNT8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GALNT8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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