Seroatlas · Human Serome Atlas

GALNT4

Polypeptide N-acetylgalactosaminyltransferase 4

Also known as: GalNAc-T4, GALT4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N4A0
Gene: GALNT4
Ensembl: ENSG00000257594
Chromosome: 12
Canonical length: 578 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Golgi apparatus

OverviewNCBI Gene

This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. In vitro, the encoded protein can complement other GalNAc-Ts in the complete O-glycosylation of the mucin-1 tandem repeat and can O-glycosylate the P-selectin glycoprotein ligand-1 molecule. The coding region of this gene is contained within a single exon. Fusion transcripts, which combine part of this gene with the 5' exons of the neighboring POC1B (POC1 centriolar protein homolog B) gene, also exist. [provided by RefSeq, Dec 2010]

Canonical amino-acid sequenceUniProt

578 residues, UniProt reviewed canonical sequence.

>Q8N4A0|GALNT4
     1  MAVRWTWAGK SCLLLAFLTV AYIFVELLVS TFHASAGAGR ARELGSRRLS DLQKNTEDLS
    61  RPLYKKPPAD SRALGEWGKA SKLQLNEDEL KQQEELIERY AINIYLSDRI SLHRHIEDKR
   121  MYECKSQKFN YRTLPTTSVI IAFYNEAWST LLRTIHSVLE TSPAVLLKEI ILVDDLSDRV
   181  YLKTQLETYI SNLDRVRLIR TNKREGLVRA RLIGATFATG DVLTFLDCHC ECNSGWLEPL
   241  LERIGRDETA VVCPVIDTID WNTFEFYMQI GEPMIGGFDW RLTFQWHSVP KQERDRRISR
   301  IDPIRSPTMA GGLFAVSKKY FQYLGTYDTG MEVWGGENLE LSFRVWQCGG KLEIHPCSHV
   361  GHVFPKRAPY ARPNFLQNTA RAAEVWMDEY KEHFYNRNPP ARKEAYGDIS ERKLLRERLR
   421  CKSFDWYLKN VFPNLHVPED RPGWHGAIRS RGISSECLDY NSPDNNPTGA NLSLFGCHGQ
   481  GGNQFFEYTS NKEIRFNSVT ELCAEVPEQK NYVGMQNCPK DGFPVPANII WHFKEDGTIF
   541  HPHSGLCLSA YRTPEGRPDV QMRTCDALDK NQIWSFEK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against GALNT4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 30 nTPM

Expression across tissuesHPA

Tissue

stomach: 30 nTPM
colon: 18 nTPM
small intestine: 17 nTPM
duodenum: 16 nTPM
rectum: 16 nTPM
cervix: 15 nTPM

Single-cell type

astrocytes: 0.1 nCPM
ependymal cells: 0.1 nCPM
microglia: 0.1 nCPM
adipocytes: 0 nCPM
adrenal cortex cells: 0 nCPM
adrenal medulla cells: 0 nCPM

Immune cell

non-classical monocyte: 5.3 nTPM
intermediate monocyte: 4.9 nTPM
NK-cell: 4.9 nTPM
basophil: 3.9 nTPM
MAIT T-cell: 3.5 nTPM
classical monocyte: 3.3 nTPM

Brain region

medulla oblongata: 4.3 nTPM
hypothalamus: 3.8 nTPM
spinal cord: 3.8 nTPM
basal ganglia: 3.7 nTPM
thalamus: 3.6 nTPM
white matter: 3.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.5
gnomAD pLI: 0.27
gnomAD missense Z: -0.01
DepMap mean gene effect: -0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads GALNT4 as an antibody target. Whether an autoantibody or antibody against GALNT4 could matter depends on whether native GALNT4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

GALNT4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label GALNT4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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