FPGS

Protein identityUniProt · HPA

UniProt accession

Q05932

Gene

FPGS

Ensembl

ENSG00000136877

Chromosome

9

Canonical length

587 aa

Protein class

Enzymes, FDA approved drug targets, Metabolic proteins, Predicted intracellular proteins

Secretome location

Intracellular and membrane

OverviewNCBI Gene

This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms. [provided by RefSeq, Jan 2014]

Canonical amino-acid sequenceUniProt

587 residues, UniProt reviewed canonical sequence.

>Q05932|FPGS
     1  MSRARSHLRA ALFLAAASAR GITTQVAARR GLSAWPVPQE PSMEYQDAVR MLNTLQTNAG
    61  YLEQVKRQRG DPQTQLEAME LYLARSGLQV EDLDRLNIIH VTGTKGKGST CAFTECILRS
   121  YGLKTGFFSS PHLVQVRERI RINGQPISPE LFTKYFWRLY HRLEETKDGS CVSMPPYFRF
   181  LTLMAFHVFL QEKVDLAVVE VGIGGAYDCT NIIRKPVVCG VSSLGIDHTS LLGDTVEKIA
   241  WQKGGIFKQG VPAFTVLQPE GPLAVLRDRA QQISCPLYLC PMLEALEEGG PPLTLGLEGE
   301  HQRSNAALAL QLAHCWLQRQ DRHGAGEPKA SRPGLLWQLP LAPVFQPTSH MRLGLRNTEW
   361  PGRTQVLRRG PLTWYLDGAH TASSAQACVR WFRQALQGRE RPSGGPEVRV LLFNATGDRD
   421  PAALLKLLQP CQFDYAVFCP NLTEVSSTGN ADQQNFTVTL DQVLLRCLEH QQHWNHLDEE
   481  QASPDLWSAP SPEPGGSASL LLAPHPPHTC SASSLVFSCI SHALQWISQG RDPIFQPPSP
   541  PKGLLTHPVA HSGASILREA AAIHVLVTGS LHLVGGVLKL LEPALSQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FPGS can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

65 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.74

gnomAD pLI

0

gnomAD missense Z

1.89

DepMap mean gene effect

-0.42

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell population proliferation
• folic acid metabolic process
• folic acid-containing compound metabolic process
• glutamate metabolic process
• nucleobase-containing compound metabolic process
• one-carbon metabolic process
• tetrahydrofolylpolyglutamate biosynthetic process

Molecular functions

• ATP binding
• metal ion binding
• tetrahydrofolylpolyglutamate synthase activity

Cellular components

• cytoplasm
• cytosol
• mitochondrial inner membrane
• mitochondrial matrix
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Folylpolyglutamate synthetase
• Folylpolyglutamate synthetase, conserved site
• Folylpolyglutamate synthase, eukaryota
• Mur-like, catalytic domain superfamily
• Mur ligase, C-terminal domain superfamily

KeywordsUniProt

• Acetylation
• Alternative initiation
• ATP-binding
• Cytoplasm
• Ligase
• Magnesium
• Membrane
• Metal-binding
• Mitochondrion
• Mitochondrion inner membrane
• Nucleotide-binding
• One-carbon metabolism
• Phosphoprotein
• Transit peptide