FADS2

Protein identityUniProt · HPA

UniProt accession

O95864

Gene

FADS2

Ensembl

ENSG00000134824

Chromosome

11

Canonical length

444 aa

Protein class

Enzymes, FDA approved drug targets, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nuclear membrane,Endoplasmic reticulum,Plasma membrane

OverviewNCBI Gene

The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

444 residues, UniProt reviewed canonical sequence.

>O95864|FADS2
     1  MGKGGNQGEG AAEREVSVPT FSWEEIQKHN LRTDRWLVID RKVYNITKWS IQHPGGQRVI
    61  GHYAGEDATD AFRAFHPDLE FVGKFLKPLL IGELAPEEPS QDHGKNSKIT EDFRALRKTA
   121  EDMNLFKTNH VFFLLLLAHI IALESIAWFT VFYFGNGWIP TLITAFVLAT SQAQAGWLQH
   181  DYGHLSVYRK PKWNHLVHKF VIGHLKGASA NWWNHRHFQH HAKPNIFHKD PDVNMLHVFV
   241  LGEWQPIEYG KKKLKYLPYN HQHEYFFLIG PPLLIPMYFQ YQIIMTMIVH KNWVDLAWAV
   301  SYYIRFFITY IPFYGILGAL LFLNFIRFLE SHWFVWVTQM NHIVMEIDQE AYRDWFSSQL
   361  TATCNVEQSF FNDWFSGHLN FQIEHHLFPT MPRHNLHKIA PLVKSLCAKH GIEYQEKPLL
   421  RALLDIIRSL KKSGKLWLDA YLHK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FADS2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

4

Mean surface accessibility (rSASA)

0.25

Highest tissue expression

303 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.23

gnomAD pLI

1

gnomAD missense Z

3.19

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 1% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• alpha-linolenic acid metabolic process
• fatty acid derivative biosynthetic process
• linoleic acid metabolic process
• lipid metabolic process
• long-chain fatty acid biosynthetic process
• unsaturated fatty acid biosynthetic process
• arachidonate metabolite production involved in inflammatory response
• positive regulation of cellular response to oxidative stress

Molecular functions

• acyl-CoA 6-desaturase activity
• oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
• stearoyl-CoA 9-desaturase activity

Cellular components

• endoplasmic reticulum membrane
• membrane
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Cytochrome b5-like heme/steroid binding domain
• Fatty acid desaturase domain
• Fatty acid desaturase
• Cytochrome b5-like heme/steroid binding domain superfamily
• Cytochrome b5-like Heme/Steroid binding domain
• Fatty acid desaturase

KeywordsUniProt

• Electron transport
• Endoplasmic reticulum
• Fatty acid biosynthesis
• Fatty acid metabolism
• Lipid biosynthesis
• Lipid metabolism
• Membrane
• Oxidoreductase
• Transmembrane
• Transmembrane helix
• Transport