Seroatlas · Human Serome Atlas

EXO5

Exonuclease V

Also known as: C1orf176, DEM1, EXO5_HUMAN, FLJ21144

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H790
Gene: EXO5
Ensembl: ENSG00000164002
Chromosome: 1
Canonical length: 373 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a single-stranded DNA (ssDNA)-specific exonuclease that can slide along the DNA before cutting it. However, human replication protein A binds ssDNA and restricts sliding of the encoded protein, providing a 5'-directionality to the enzyme. This protein localizes to nuclear repair loci after DNA damage. [provided by RefSeq, Nov 2016]

Canonical amino-acid sequenceUniProt

373 residues, UniProt reviewed canonical sequence.

>Q9H790|EXO5
     1  MAETREEETV SAEASGFSDL SDSEFLEFLD LEDAQESKAL VNMPGPSSES LGKDDKPISL
    61  QNWKRGLDIL SPMERFHLKY LYVTDLATQN WCELQTAYGK ELPGFLAPEK AAVLDTGASI
   121  HLARELELHD LVTVPVTTKE DAWAIKFLNI LLLIPTLQSE GHIREFPVFG EGEGVLLVGV
   181  IDELHYTAKG ELELAELKTR RRPMLPLEAQ KKKDCFQVSL YKYIFDAMVQ GKVTPASLIH
   241  HTKLCLEKPL GPSVLRHAQQ GGFSVKSLGD LMELVFLSLT LSDLPVIDIL KIEYIHQETA
   301  TVLGTEIVAF KEKEVRAKVQ HYMAYWMGHR EPQGVDVEEA WKCRTCTYAD ICEWRKGSGV
   361  LSSTLAPQVK KAK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EXO5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 8.5 nTPM

Expression across tissuesHPA

Tissue

tonsil: 8.5 nTPM
thymus: 8.2 nTPM
lymph node: 6.7 nTPM
spleen: 6.2 nTPM
smooth muscle: 5.9 nTPM
liver: 5.3 nTPM

Single-cell type

tuft cells: 12 nCPM
erythrocyte progenitors: 8.9 nCPM
early primary spermatocytes: 8.5 nCPM
hofbauer cells: 6.8 nCPM
megakaryocytes: 6.2 nCPM
cholangiocytes: 5.9 nCPM

Immune cell

eosinophil: 12 nTPM
T-reg: 10 nTPM
gdT-cell: 10 nTPM
MAIT T-cell: 9.1 nTPM
memory B-cell: 9.1 nTPM
naive CD8 T-cell: 8.9 nTPM

Brain region

cerebellum: 8.3 nTPM
basal ganglia: 7.4 nTPM
cerebral cortex: 6.6 nTPM
hypothalamus: 6.5 nTPM
white matter: 5.7 nTPM
amygdala: 5.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.43
gnomAD pLI: 0
gnomAD missense Z: 0.95
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

interstrand cross-link repair

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

PD-(D/E)XK endonuclease-like domain superfamily
Exonuclease V
Exonuclease V - a 5' deoxyribonuclease

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EXO5 as an antibody target. Whether an autoantibody or antibody against EXO5 could matter depends on whether native EXO5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EXO5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EXO5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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