Seroatlas · Human Serome Atlas

EPHX4

Epoxide hydrolase 4

Also known as: ABHD7, EH4, EPHX4_HUMAN, EPHXRP, FLJ90341

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IUS5
Gene: EPHX4
Ensembl: ENSG00000172031
Chromosome: 1
Canonical length: 362 aa
Protein class: Predicted intracellular proteins
Subcellular location: Vesicles

OverviewNCBI Gene

Predicted to enable hydrolase activity. Predicted to be located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

362 residues, UniProt reviewed canonical sequence.

>Q8IUS5|EPHX4
     1  MARLRDCLPR LMLTLRSLLF WSLVYCYCGL CASIHLLKLL WSLGKGPAQT FRRPAREHPP
    61  ACLSDPSLGT HCYVRIKDSG LRFHYVAAGE RGKPLMLLLH GFPEFWYSWR YQLREFKSEY
   121  RVVALDLRGY GETDAPIHRQ NYKLDCLITD IKDILDSLGY SKCVLIGHDW GGMIAWLIAI
   181  CYPEMVMKLI VINFPHPNVF TEYILRHPAQ LLKSSYYYFF QIPWFPEFMF SINDFKVLKH
   241  LFTSHSTGIG RKGCQLTTED LEAYIYVFSQ PGALSGPINH YRNIFSCLPL KHHMVTTPTL
   301  LLWGENDAFM EVEMAEVTKI YVKNYFRLTI LSEASHWLQQ DQPDIVNKLI WTFLKEETRK
   361  KD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EPHX4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 14 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 14 nTPM
basal ganglia: 10 nTPM
choroid plexus: 6.6 nTPM
amygdala: 4.1 nTPM
hippocampal formation: 2.6 nTPM
midbrain: 2.3 nTPM

Single-cell type

retinal amacrine cells: 42 nCPM
brain inhibitory neurons: 37 nCPM
retinal ganglion cells: 23 nCPM
brain excitatory neurons: 20 nCPM
endometrial secretory cells: 13 nCPM
smooth muscle cells: 12 nCPM

Immune cell

gdT-cell: 12 nTPM
memory CD8 T-cell: 3 nTPM
naive CD8 T-cell: 2.8 nTPM
MAIT T-cell: 2 nTPM
total PBMC: 1.5 nTPM
memory CD4 T-cell: 1.4 nTPM

Brain region

cerebral cortex: 22 nTPM
basal ganglia: 19 nTPM
choroid plexus: 13 nTPM
white matter: 12 nTPM
amygdala: 7.9 nTPM
pons: 6.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.91
gnomAD pLI: 0
gnomAD missense Z: 1.06
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EPHX4 as an antibody target. Whether an autoantibody or antibody against EPHX4 could matter depends on whether native EPHX4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EPHX4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EPHX4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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