Seroatlas · Human Serome Atlas

EPHX2

Bifunctional epoxide hydrolase 2

Also known as: ABHD20, HYES_HUMAN, sEH

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P34913
Gene
EPHX2
Ensembl
ENSG00000120915
Chromosome
8
Canonical length
555 aa
Protein class
Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location
Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012]

Canonical amino-acid sequenceUniProt

555 residues, UniProt reviewed canonical sequence.

>P34913|EPHX2
     1  MTLRAAVFDL DGVLALPAVF GVLGRTEEAL ALPRGLLNDA FQKGGPEGAT TRLMKGEITL
    61  SQWIPLMEEN CRKCSETAKV CLPKNFSIKE IFDKAISARK INRPMLQAAL MLRKKGFTTA
   121  ILTNTWLDDR AERDGLAQLM CELKMHFDFL IESCQVGMVK PEPQIYKFLL DTLKASPSEV
   181  VFLDDIGANL KPARDLGMVT ILVQDTDTAL KELEKVTGIQ LLNTPAPLPT SCNPSDMSHG
   241  YVTVKPRVRL HFVELGSGPA VCLCHGFPES WYSWRYQIPA LAQAGYRVLA MDMKGYGESS
   301  APPEIEEYCM EVLCKEMVTF LDKLGLSQAV FIGHDWGGML VWYMALFYPE RVRAVASLNT
   361  PFIPANPNMS PLESIKANPV FDYQLYFQEP GVAEAELEQN LSRTFKSLFR ASDESVLSMH
   421  KVCEAGGLFV NSPEEPSLSR MVTEEEIQFY VQQFKKSGFR GPLNWYRNME RNWKWACKSL
   481  GRKILIPALM VTAEKDFVLV PQMSQHMEDW IPHLKRGHIE DCGHWTQMDK PTEVNQILIK
   541  WLDSDARNPP VVSKM

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EPHX2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.23
Highest tissue expression
311 nTPM

Expression across tissuesHPA

Tissue

  • liver: 311 nTPM
  • kidney: 155 nTPM
  • small intestine: 121 nTPM
  • duodenum: 106 nTPM
  • choroid plexus: 88 nTPM
  • adrenal gland: 67 nTPM

Single-cell type

  • enterocytes: 378 nCPM
  • adrenal cortex cells: 224 nCPM
  • hepatocytes: 166 nCPM
  • epididymal efferent duct absorptive cells: 140 nCPM
  • proximal tubule cells: 140 nCPM
  • paneth cells: 124 nCPM

Immune cell

  • naive CD4 T-cell: 107 nTPM
  • naive CD8 T-cell: 51 nTPM
  • memory CD4 T-cell: 35 nTPM
  • T-reg: 30 nTPM
  • total PBMC: 20 nTPM
  • memory CD8 T-cell: 13 nTPM

Brain region

  • choroid plexus: 71 nTPM
  • midbrain: 19 nTPM
  • medulla oblongata: 17 nTPM
  • spinal cord: 16 nTPM
  • thalamus: 15 nTPM
  • white matter: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.26
gnomAD pLI
0
gnomAD missense Z
0.24
DepMap mean gene effect
0
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EPHX2 as an antibody target. Whether an autoantibody or antibody against EPHX2 could matter depends on whether native EPHX2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EPHX2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EPHX2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/EPHX2. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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