Seroatlas · Human Serome Atlas

ENTPD4

Ectonucleoside triphosphate diphosphohydrolase 4

Also known as: ENTP4_HUMAN, KIAA0392, LALP70, LAP70, LYSAL1, NTPDase-4, UDPase

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y227
Gene
ENTPD4
Ensembl
ENSG00000197217
Chromosome
8
Canonical length
616 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Vesicles

OverviewNCBI Gene

This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011]

Canonical amino-acid sequenceUniProt

616 residues, UniProt reviewed canonical sequence.

>Q9Y227|ENTPD4
     1  MGRIGISCLF PASWHFSISP VGCPRILNTN LRQIMVISVL AAAVSLLYFS VVIIRNKYGR
    61  LTRDKKFQRY LARVTDIEAT DTNNPNVNYG IVVDCGSSGS RVFVYCWPRH NGNPHDLLDI
   121  RQMRDKNRKP VVMKIKPGIS EFATSPEKVS DYISPLLNFA AEHVPRAKHK ETPLYILCTA
   181  GMRILPESQQ KAILEDLLTD IPVHFDFLFS DSHAEVISGK QEGVYAWIGI NFVLGRFEHI
   241  EDDDEAVVEV NIPGSESSEA IVRKRTAGIL DMGGVSTQIA YEVPKTVSFA SSQQEEVAKN
   301  LLAEFNLGCD VHQTEHVYRV YVATFLGFGG NAARQRYEDR IFANTIQKNR LLGKQTGLTP
   361  DMPYLDPCLP LDIKDEIQQN GQTIYLRGTG DFDLCRETIQ PFMNKTNETQ TSLNGVYQPP
   421  IHFQNSEFYG FSEFYYCTED VLRMGGDYNA AKFTKAAKDY CATKWSILRE RFDRGLYASH
   481  ADLHRLKYQC FKSAWMFEVF HRGFSFPVNY KSLKTALQVY DKEVQWTLGA ILYRTRFLPL
   541  RDIQQEAFRA SHTHWRGVSF VYNHYLFSGC FLVVLLAILL YLLRLRRIHR RTPRSSSAAA
   601  LWMEEGLPAQ NAPGTL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENTPD4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
2
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
30 nTPM

Expression across tissuesHPA

Tissue

  • retina: 30 nTPM
  • bone marrow: 29 nTPM
  • tonsil: 28 nTPM
  • lymph node: 27 nTPM
  • parathyroid gland: 26 nTPM
  • small intestine: 25 nTPM

Single-cell type

  • neutrophils: 117 nCPM
  • lactotrophs: 80 nCPM
  • neutrophil progenitors: 69 nCPM
  • plasma cells: 68 nCPM
  • somatotrophs: 68 nCPM
  • b-cells: 68 nCPM

Immune cell

  • basophil: 17 nTPM
  • neutrophil: 14 nTPM
  • eosinophil: 13 nTPM
  • naive CD4 T-cell: 9.3 nTPM
  • MAIT T-cell: 8.8 nTPM
  • memory B-cell: 8.8 nTPM

Brain region

  • cerebral cortex: 48 nTPM
  • medulla oblongata: 43 nTPM
  • pons: 42 nTPM
  • hypothalamus: 41 nTPM
  • thalamus: 41 nTPM
  • cerebellum: 39 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.02
gnomAD pLI
0
gnomAD missense Z
-0.72
DepMap mean gene effect
0.03
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ENTPD4 as an antibody target. Whether an autoantibody or antibody against ENTPD4 could matter depends on whether native ENTPD4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ENTPD4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ENTPD4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/ENTPD4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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