Seroatlas · Human Serome Atlas

ENGASE

Cytosolic endo-beta-N-acetylglucosaminidase

Also known as: ENASE_HUMAN, FLJ21865

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8NFI3
Gene: ENGASE
Ensembl: ENSG00000167280
Chromosome: 17
Canonical length: 743 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location: Centrosome,Cytosol

OverviewNCBI Gene

This gene encodes a cytosolic enzyme which catalyzes the hydrolysis of peptides and proteins with mannose modifications to produce free oligosaccharides. [provided by RefSeq, Feb 2012]

Canonical amino-acid sequenceUniProt

743 residues, UniProt reviewed canonical sequence.

>Q8NFI3|ENGASE
     1  MEAAAVTVTR SATRRRRRQL QGLAAPEAGT QEEQEDQEPR PRRRRPGRSI KDEEEETVFR
    61  EVVSFSPDPL PVRYYDKDTT KPISFYLSSL EELLAWKPRL EDGFNVALEP LACRQPPLSS
   121  QRPRTLLCHD MMGGYLDDRF IQGSVVQTPY AFYHWQCIDV FVYFSHHTVT IPPVGWTNTA
   181  HRHGVCVLGT FITEWNEGGR LCEAFLAGDE RSYQAVADRL VQITQFFRFD GWLINIENSL
   241  SLAAVGNMPP FLRYLTTQLH RQVPGGLVLW YDSVVQSGQL KWQDELNQHN RVFFDSCDGF
   301  FTNYNWREEH LERMLGQAGE RRADVYVGVD VFARGNVVGG RFDTDKSLEL IRKHGFSVAL
   361  FAPGWVYECL EKKDFFQNQD KFWGRLERYL PTHSICSLPF VTSFCLGMGA RRVCYGQEEA
   421  VGPWYHLSAQ EIQPLFGEHR LGGDGRGWVR THCCLEDAWH GGSSLLVRGV IPPEVGNVAV
   481  RLFSLQAPVP PKIYLSMVYK LEGPTDVTVA LELTTGDAGS CHIGGISVLN AETSSRHSLR
   541  PLRVPPTKLA RWVGRCGRQL SGGWVQHCYE VSLRGCLLLD LLVCFSRPPG SREEESFTCR
   601  LGEIQVVDAA SLLAPLPQVQ AVTISHIRWQ PSASEREGPP ALLQLSCTLH WSFLLSQVRC
   661  FRIHCWGGMS DDSPGRELPR PEMPMFLGLA FATQYRIVDL LVEAAGPGQD RRMEFLVEPV
   721  PKEGFRVPQA EWGRAVLLYS APA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ENGASE can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 22 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 22 nTPM
spleen: 21 nTPM
ovary: 17 nTPM
colon: 17 nTPM
small intestine: 16 nTPM
urinary bladder: 15 nTPM

Single-cell type

myonuclei: 68 nCPM
microglia: 31 nCPM
colonocytes: 28 nCPM
enteric stem cells: 28 nCPM
macrophages: 26 nCPM
adrenal cortex cells: 25 nCPM

Immune cell

T-reg: 0.7 nTPM
naive CD4 T-cell: 0.5 nTPM
myeloid DC: 0.4 nTPM
NK-cell: 0.4 nTPM
classical monocyte: 0.3 nTPM
MAIT T-cell: 0.2 nTPM

Brain region

white matter: 21 nTPM
cerebral cortex: 21 nTPM
thalamus: 19 nTPM
midbrain: 18 nTPM
choroid plexus: 17 nTPM
pons: 17 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.34
gnomAD pLI: 0
gnomAD missense Z: -0.02
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity

Cellular components

cytosol

Protein domainsUniProt · Pfam · InterPro

BRCT domain
Cytosolic endo-beta-N-acetylglucosaminidase, TIM barrel domain
Cytosolic endo-beta-N-acetylglucosaminidase
Cytosolic endo-beta-N-acetylglucosaminidase, C-terminal domain
Glycosyl hydrolase family 85
ENGASE1-like, C-terminal immunoglobulin-like domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ENGASE as an antibody target. Whether an autoantibody or antibody against ENGASE could matter depends on whether native ENGASE is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ENGASE is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ENGASE as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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