Seroatlas · Human Serome Atlas

EME2

Structure-specific endonuclease subunit EME2

Also known as: EME2_HUMAN, FLJ00151, SLX2B

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A4GXA9
Gene: EME2
Ensembl: ENSG00000197774
Chromosome: 16
Canonical length: 379 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

EME2 forms a heterodimer with MUS81 (MIM 606591) that functions as an XPF (MIM 278760)-type flap/fork endonuclease in DNA repair (Ciccia et al., 2007 [PubMed 17289582]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

379 residues, UniProt reviewed canonical sequence.

>A4GXA9|EME2
     1  MARVGPGRAG VSCQGRGRGR GGSGQRRPPT WEISDSDAED SAGSEAAARA RDPAGERRAA
    61  AEALRLLRPE QVLKRLAVCV DTAILEDAGA DVLMEALEAL GCECRIEPQR PARSLRWTRA
   121  SPDPCPRSLP PEVWAAGEQE LLLLLEPEEF LQGVATLTQI SGPTHWVPWI SPETTARPHL
   181  AVIGLDAYLW SRQHVSRGTQ QPESPKVAGA EVAVSWPEVE EALVLLQLWA NLDVLLVASW
   241  QELSRHVCAV TKALAQYPLK QYRESQAFSF CTAGRWAAGE PVARDGAGLQ AAWRRQIRQF
   301  SRVSPAVADA VVTAFPSPRL LQQALEACST ERERMGLLAD LPVPPSEGGR PRRVGPDLSR
   361  RICLFLTTAN PDLLLDLGS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EME2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 11 nTPM

Expression across tissuesHPA

Tissue

pituitary gland: 11 nTPM
cerebellum: 10 nTPM
prostate: 6 nTPM
spleen: 5.1 nTPM
thyroid gland: 4.9 nTPM
esophagus: 4.1 nTPM

Single-cell type

cardiomyocytes: 811 nCPM
epicardial cells: 366 nCPM
adipocytes: 116 nCPM
fibroblasts: 39 nCPM
plasma cells: 23 nCPM
other brain neurons: 23 nCPM

Immune cell

naive B-cell: 0.4 nTPM
plasmacytoid DC: 0.4 nTPM
classical monocyte: 0.3 nTPM
intermediate monocyte: 0.3 nTPM
memory B-cell: 0.3 nTPM
memory CD8 T-cell: 0.3 nTPM

Brain region

cerebral cortex: 8.3 nTPM
cerebellum: 7.7 nTPM
hippocampal formation: 7.1 nTPM
choroid plexus: 6.6 nTPM
pons: 6.5 nTPM
basal ganglia: 6.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.94
gnomAD pLI: 0
gnomAD missense Z: -1.78
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

ERCC4 domain
Mms4/EME1/EME2
EME1/EME2, C-terminal domain
ERCC4 domain
EME1/MUS81, C-terminal
Crossover junction endonuclease EME2, XPF-like nuclease domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EME2 as an antibody target. Whether an autoantibody or antibody against EME2 could matter depends on whether native EME2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EME2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EME2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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