EGR4
Early growth response protein 4
Also known as: EGR4_HUMAN, NGFI-C, PAT133
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q05215
- Gene
- EGR4
- Ensembl
- ENSG00000135625
- Chromosome
- 2
- Canonical length
- 589 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
OverviewNCBI Gene
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and sequence-specific double-stranded DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
589 residues, UniProt reviewed canonical sequence.
>Q05215|EGR4
1 MAVARGVGSP EPAPPQLYKW GGCGLGEPGS ALERRGAAAR GRCGRARAPR LPDSFPRGEC
61 PKPGARAPRS VRCGEPLPPA SPPPARPQAQ RARPRAPHSR RRAMLHLSEF SEPDALLVKS
121 TEGCCAEPSA ELPRLPARDA PAATGYPGAG DFLSWALNSC GASGDLADSC FLEGPAPTPP
181 PGLSYSGSFF IQAVPEHPHD PEALFNLMSG ILGLAPFPGP EAAASRSPLD APFPAGSDAL
241 LPGPPDLYSP DLGAAPFPEA FWEASPCAGA PSQCLYEPQL SPPDVKPGLR APPASPALDA
301 VSAFKGPYAP WELLSVGAPG NCGSQGDYQA APEARFPVIG TKIEDLLSIS CPAELPAVPA
361 NRLYPSGAYD AFPLAPGDLG EGAEGLPGLL TPPSGEGGSS GDGGEFLAST QPQLSPLGLR
421 SAAAADFPKP LVADIPGSSG VAAPPVPPPP PTPFPQAKAR RKGRRGGKCS TRCFCPRPHA
481 KAFACPVESC VRSFARSDEL NRHLRIHTGH KPFQCRICLR NFSRSDHLTT HVRTHTGEKP
541 FACDVCGRRF ARSDEKKRHS KVHLKQKARA EERLKGLGFY SLGLSFASLLocalizationUniProt · AlphaFold · HPA
Whether an antibody against EGR4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.67
- Highest tissue expression
- 19 nTPM
Expression across tissuesHPA
Tissue
- cerebellum: 19 nTPM
- basal ganglia: 12 nTPM
- hippocampal formation: 11 nTPM
- cerebral cortex: 7.2 nTPM
- amygdala: 5.3 nTPM
- testis: 3.9 nTPM
Single-cell type
- granulosa cells: 58 nCPM
- neuroendocrine cells: 48 nCPM
- breast secretory cells: 25 nCPM
- undifferentiated spermatogonia: 23 nCPM
- endometrial glandular cells: 21 nCPM
- epididymal basal cells: 18 nCPM
Immune cell
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
- MAIT T-cell: 0 nTPM
Brain region
- cerebral cortex: 113 nTPM
- cerebellum: 68 nTPM
- hippocampal formation: 49 nTPM
- basal ganglia: 15 nTPM
- white matter: 8.9 nTPM
- amygdala: 8.3 nTPM
DiseaseUniProt · ClinVar · IEDB · PubMed
Four sources answering four different questions about EGR4.
Disease | ImmuneIEDB
Conditions an epitope on EGR4 was assayed in.
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.02
- gnomAD pLI
- 0.01
- gnomAD missense Z
- -0.14
- DepMap mean gene effect
- -0.05
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- positive regulation of cell population proliferation
- positive regulation of transcription by RNA polymerase II
- regulation of transcription by RNA polymerase II
Molecular functions
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
- zinc ion binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads EGR4 as an antibody target. Whether an autoantibody or antibody against EGR4 could matter depends on whether native EGR4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
EGR4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label EGR4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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