Seroatlas · Human Serome Atlas

EEF1AKMT1

EEF1A lysine methyltransferase 1

Also known as: EFMT1_HUMAN, N6AMT2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8WVE0
Gene: EEF1AKMT1
Ensembl: ENSG00000150456
Chromosome: 13
Canonical length: 214 aa
Protein class: Predicted intracellular proteins
Subcellular location: Plasma membrane,Cytosol

OverviewNCBI Gene

Enables protein-lysine N-methyltransferase activity. Involved in peptidyl-lysine methylation. Predicted to be located in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

214 residues, UniProt reviewed canonical sequence.

>Q8WVE0|EEF1AKMT1
     1  MSDLEDDETP QLSAHALAAL QEFYAEQKQQ IEPGEDDKYN IGIIEENWQL SQFWYSQETA
    61  LQLAQEAIAA VGEGGRIACV SAPSVYQKLR ELCRENFSIY IFEYDKRFAM YGEEFIFYDY
   121  NNPLDLPERI AAHSFDIVIA DPPYLSEECL RKTSETVKYL TRGKILLCTG AIMEEQAAEL
   181  LGVKMCTFVP RHTRNLANEF RCYVNYDSGL DCGI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EEF1AKMT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.31
Highest tissue expression: 14 nTPM

Expression across tissuesHPA

Tissue

epididymis: 14 nTPM
choroid plexus: 13 nTPM
amygdala: 13 nTPM
hypothalamus: 13 nTPM
midbrain: 13 nTPM
liver: 13 nTPM

Single-cell type

myonuclei: 96 nCPM
oocytes: 60 nCPM
decidual stromal cells: 47 nCPM
bergmann glia: 45 nCPM
myosatellite cells: 44 nCPM
undifferentiated spermatogonia: 41 nCPM

Immune cell

NK-cell: 16 nTPM
memory B-cell: 13 nTPM
naive CD4 T-cell: 13 nTPM
T-reg: 12 nTPM
naive CD8 T-cell: 11 nTPM
plasmacytoid DC: 11 nTPM

Brain region

hypothalamus: 13 nTPM
white matter: 12 nTPM
spinal cord: 12 nTPM
medulla oblongata: 12 nTPM
cerebral cortex: 12 nTPM
cerebellum: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.57
gnomAD pLI: 0
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

peptidyl-lysine methylation

Molecular functions

Cellular components

cytosol

Protein domainsUniProt · Pfam · InterPro

DNA methylase, N-6 adenine-specific, conserved site
S-adenosyl-L-methionine-dependent methyltransferase superfamily
Methyltransferase EEF1AKMT1/ZCCHC4
Probable N6-adenine methyltransferase
Protein-lysine N-methyltransferase Efm5/EEF1AKMT1

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EEF1AKMT1 as an antibody target. Whether an autoantibody or antibody against EEF1AKMT1 could matter depends on whether native EEF1AKMT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EEF1AKMT1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label EEF1AKMT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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