EDF1

Protein identityUniProt · HPA

UniProt accession

O60869

Gene

EDF1

Ensembl

ENSG00000107223

Chromosome

9

Canonical length

148 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a protein that may regulate endothelial cell differentiation, lipid metabolism, and hormone-induced cardiomyocyte hypertrophy. The encoded protein has also been found to act as a transcriptional coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

148 residues, UniProt reviewed canonical sequence.

>O60869|EDF1
     1  MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK QHSITKNTAK
    61  LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN EKPQVIADYE SGRAIPNNQV
   121  LGKIERAIGL KLRGKDIGKP IEKGPRAK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EDF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.45

Highest tissue expression

677 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.51

gnomAD pLI

0.78

gnomAD missense Z

1.56

DepMap mean gene effect

-0.23

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• endothelial cell differentiation
• positive regulation of DNA binding
• positive regulation of DNA-templated transcription
• regulation of DNA-templated transcription
• regulation of lipid metabolic process

Molecular functions

• calmodulin binding
• DNA binding
• RNA binding
• TFIID-class transcription factor complex binding
• transcription coactivator activity

Cellular components

• cytoplasm
• cytosol
• nucleolus
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Cro/C1-type, helix-turn-helix domain
• Lambda repressor-like, DNA-binding domain superfamily
• Multiprotein bridging factor 1, N-terminal
• Helix-turn-helix
• Multiprotein bridging factor 1

KeywordsUniProt

• Acetylation
• Activator
• Calmodulin-binding
• Cytoplasm
• Developmental protein
• Differentiation
• DNA-binding
• Methylation
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of EDF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TBP
• NR1H3
• PPARG