EDDM3B
Epididymal secretory protein E3-beta
Also known as: EP3B_HUMAN, FAM12B, HE3-BETA
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P56851
- Gene
- EDDM3B
- Ensembl
- ENSG00000181552
- Chromosome
- 14
- Canonical length
- 147 aa
- Protein class
- Predicted secreted proteins
- Secretome location
- Secreted in male reproductive system
OverviewNCBI Gene
Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
147 residues, UniProt reviewed canonical sequence.
>P56851|EDDM3B
1 MASSLKIWGT LLALLCILCT LLVQSKEVSW REFMKQHYLS PSREFREYKC DVLMRENEAL
61 KDKSSHMFIY ISWYKIEHIC TSDNWMDRFR NAYVWVQNPL KVLKCHQENS KNSYTESRSF
121 NYIEFHCSMD GYVDSIEDLK MVEPIGNLocalizationUniProt · AlphaFold · HPA
Whether an antibody against EDDM3B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Secreted
- Secreted
- Yes
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.34
- Highest tissue expression
- 2,420 nTPM
Expression across tissuesHPA
Tissue
- epididymis: 2,420 nTPM
- seminal vesicle: 8.6 nTPM
- breast: 0.2 nTPM
- adipose tissue: 0 nTPM
- adrenal gland: 0 nTPM
- amygdala: 0 nTPM
Single-cell type
- epididymal principal cells: 3,065 nCPM
- epididymal clear cells: 35 nCPM
- epididymal basal cells: 31 nCPM
- mast cells: 11 nCPM
- t-cells: 4 nCPM
- vascular smooth muscle cells: 2.8 nCPM
Immune cell
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
- MAIT T-cell: 0 nTPM
Brain region
- hypothalamus: 0.9 nTPM
- white matter: 0.9 nTPM
- basal ganglia: 0.8 nTPM
- medulla oblongata: 0.7 nTPM
- thalamus: 0.7 nTPM
- midbrain: 0.6 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD missense Z
- 0.13
- DepMap mean gene effect
- -0.06
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 2% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads EDDM3B as an antibody target. Whether an autoantibody or antibody against EDDM3B could matter depends on whether native EDDM3B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
EDDM3B is annotated as secreted, so native EDDM3B circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.
Annotation status
The present source text does not explicitly label EDDM3B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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