Seroatlas · Human Serome Atlas

EDDM3A

Epididymal secretory protein E3-alpha

Also known as: EP3A_HUMAN, FAM12A, HE3-ALPHA

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q14507
Gene: EDDM3A
Ensembl: ENSG00000181562
Chromosome: 14
Canonical length: 147 aa
Protein class: Predicted secreted proteins
Secretome location: Secreted in male reproductive system

OverviewNCBI Gene

Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

147 residues, UniProt reviewed canonical sequence.

>Q14507|EDDM3A
     1  MTSSLKIWGI LLALLCILCR LCVYSNNIYW REFIKLHYLS PSREFKEYKC DVLMREKEAL
    61  KGKSFHMFIY SLWFKIQRAC INEKGSDRYR NAYVWAPGAL KVLECHWEKY NNRYTESRSF
   121  SYIEFHCGVD GYVDNIEDLR IIEPISN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EDDM3A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Secreted
Secreted: Yes
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 601 nTPM

Expression across tissuesHPA

Tissue

epididymis: 601 nTPM
seminal vesicle: 33 nTPM
testis: 1.1 nTPM
kidney: 0.7 nTPM
choroid plexus: 0.2 nTPM
prostate: 0.2 nTPM

Single-cell type

epididymal principal cells: 714 nCPM
epididymal clear cells: 8.2 nCPM
epididymal basal cells: 5.4 nCPM
mast cells: 2.5 nCPM
renal collecting duct principal cells: 2.5 nCPM
cardiomyocytes: 2.3 nCPM

Immune cell

naive B-cell: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

hypothalamus: 1.2 nTPM
white matter: 0.8 nTPM
basal ganglia: 0.7 nTPM
spinal cord: 0.6 nTPM
thalamus: 0.6 nTPM
medulla oblongata: 0.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD missense Z: -0.11
DepMap mean gene effect: 0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

sperm displacement

Cellular components

extracellular space

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads EDDM3A as an antibody target. Whether an autoantibody or antibody against EDDM3A could matter depends on whether native EDDM3A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

EDDM3A is annotated as secreted, so native EDDM3A circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label EDDM3A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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