Seroatlas · Human Serome Atlas

ECHDC3

Enoyl-CoA hydratase domain-containing protein 3, mitochondrial

Also known as: ECHD3_HUMAN, FLJ20909

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96DC8
Gene: ECHDC3
Ensembl: ENSG00000134463
Chromosome: 10
Canonical length: 303 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable enoyl-CoA hydratase activity. Involved in positive regulation of cellular response to insulin stimulus. Located in mitochondrion. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

303 residues, UniProt reviewed canonical sequence.

>Q96DC8|ECHDC3
     1  MAAVAVLRAF GASGPMCLRR GPWAQLPARF CSRDPAGAGR RESEPRPTSA RQLDGIRNIV
    61  LSNPKKRNAL SLAMLKSLQS DILHDADSND LKVIIISAEG PVFSSGHDLK ELTEEQGRDY
   121  HAEVFQTCSK VMMHIRNHPV PVIAMVNGLA AAAGCQLVAS CDIAVASDKS SFATPGVNVG
   181  LFCSTPGVAL ARAVPRKVAL EMLFTGEPIS AQEALLHGLL SKVVPEAELQ EETMRIARKI
   241  ASLSRPVVSL GKATFYKQLP QDLGTAYYLT SQAMVDNLAL RDGQEGITAF LQKRKPVWSH
   301  EPV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ECHDC3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 158 nTPM

Expression across tissuesHPA

Tissue

liver: 158 nTPM
skeletal muscle: 123 nTPM
adipose tissue: 67 nTPM
heart muscle: 64 nTPM
kidney: 62 nTPM
tongue: 56 nTPM

Single-cell type

thymic myoid cells: 407 nCPM
neutrophils: 218 nCPM
hepatocytes: 173 nCPM
esophageal apical cells: 120 nCPM
cardiomyocytes: 108 nCPM
cytotrophoblasts: 105 nCPM

Immune cell

eosinophil: 16 nTPM
neutrophil: 3.6 nTPM
basophil: 3 nTPM
classical monocyte: 1.8 nTPM
myeloid DC: 1.7 nTPM
naive B-cell: 1.7 nTPM

Brain region

choroid plexus: 34 nTPM
hypothalamus: 6.5 nTPM
white matter: 4.5 nTPM
medulla oblongata: 4.2 nTPM
spinal cord: 3.8 nTPM
midbrain: 3.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.74
gnomAD pLI: 0
gnomAD missense Z: -0.19
DepMap mean gene effect: 0.07
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

mitochondrion

Protein domainsUniProt · Pfam · InterPro

Enoyl-CoA hydratase/isomerase-like domain
Enoyl-CoA hydratase, C-terminal
ClpP/crotonase-like domain superfamily
Enoyl-CoA hydratase/isomerase
Mitochondrial enoyl-CoA hydratase domain-containing protein

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ECHDC3 as an antibody target. Whether an autoantibody or antibody against ECHDC3 could matter depends on whether native ECHDC3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ECHDC3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ECHDC3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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