DUSP8

Protein identityUniProt · HPA

UniProt accession

Q13202

Gene

DUSP8

Ensembl

ENSG00000184545

Chromosome

11

Canonical length

625 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

625 residues, UniProt reviewed canonical sequence.

>Q13202|DUSP8
     1  MAGDRLPRKV MDAKKLASLL RGGPGGPLVI DSRSFVEYNS WHVLSSVNIC CSKLVKRRLQ
    61  QGKVTIAELI QPAARSQVEA TEPQDVVVYD QSTRDASVLA ADSFLSILLS KLDGCFDSVA
   121  ILTGGFATFS SCFPGLCEGK PAALLPMSLS QPCLPVPSVG LTRILPHLYL GSQKDVLNKD
   181  LMTQNGISYV LNASNSCPKP DFICESRFMR VPINDNYCEK LLPWLDKSIE FIDKAKLSSC
   241  QVIVHCLAGI SRSATIAIAY IMKTMGMSSD DAYRFVKDRR PSISPNFNFL GQLLEYERSL
   301  KLLAALQGDP GTPSGTPEPP PSPAAGAPLP RLPPPTSESA ATGNAAAREG GLSAGGEPPA
   361  PPTPPATSAL QQGLRGLHLS SDRLQDTNRL KRSFSLDIKS AYAPSRRPDG PGPPDPGEAP
   421  KLCKLDSPSG AALGLSSPSP DSPDAAPEAR PRPRRRPRPP AGSPARSPAH SLGLNFGDAA
   481  RQTPRHGLSA LSAPGLPGPG QPAGPGAWAP PLDSPGTPSP DGPWCFSPEG AQGAGGVLFA
   541  PFGRAGAPGP GGGSDLRRRE AARAEPRDAR TGWPEEPAPE TQFKRRSCQM EFEEGMVEGR
   601  ARGEELAALG KQASFSGSVE VIEVS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DUSP8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.52

Highest tissue expression

44 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about DUSP8.

Disease | ImmuneIEDB

Conditions an epitope on DUSP8 was assayed in.

• narcolepsy B cell
• multiple sclerosis B cell
• peripheral nervous system disease B cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.5

gnomAD pLI

0.7

gnomAD missense Z

2.33

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• dephosphorylation
• negative regulation of MAPK cascade
• signal transduction

Molecular functions

• MAP kinase tyrosine phosphatase activity
• MAP kinase tyrosine/serine/threonine phosphatase activity
• phosphatase activity
• protein serine/threonine phosphatase activity
• protein tyrosine/threonine phosphatase activity

Cellular components

• cytoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Dual specificity phosphatase, catalytic domain
• Tyrosine-specific protein phosphatases domain
• Rhodanese-like domain
• Mitogen-activated protein (MAP) kinase phosphatase
• Protein-tyrosine phosphatase, active site
• Dual specificity protein phosphatase domain
• Protein-tyrosine phosphatase-like
• Rhodanese-like domain superfamily
• Rhodanese-like domain
• Dual specificity phosphatase, catalytic domain
• Dual specificity protein phosphatase 8, dual specificity phosphatase domain

KeywordsUniProt

• Cytoplasm
• Hydrolase
• Nucleus
• Protein phosphatase

InteractionsUniProt · HPA

Protein binding partners of DUSP8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MAPK8