DUSP3
Dual specificity protein phosphatase 3
Also known as: DUS3_HUMAN, VHR
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P51452
- Gene
- DUSP3
- Ensembl
- ENSG00000108861
- Chromosome
- 17
- Canonical length
- 185 aa
- Protein class
- Enzymes, Plasma proteins, Predicted intracellular proteins
- Subcellular location
- Nucleoplasm,Cytosol
OverviewNCBI Gene
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq, Jul 2008]
Canonical amino-acid sequenceUniProt
185 residues, UniProt reviewed canonical sequence.
>P51452|DUSP3
1 MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL
61 NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV
121 LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE
181 GKLKPLocalizationUniProt · AlphaFold · HPA
Whether an antibody against DUSP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.26
- Highest tissue expression
- 245 nTPM
Expression across tissuesHPA
Tissue
- skeletal muscle: 245 nTPM
- tongue: 199 nTPM
- heart muscle: 185 nTPM
- liver: 105 nTPM
- colon: 92 nTPM
- blood vessel: 83 nTPM
Single-cell type
- late spermatids: 288 nCPM
- esophageal apical cells: 200 nCPM
- hofbauer cells: 143 nCPM
- hepatocytes: 121 nCPM
- early spermatids: 101 nCPM
- alveolar cells type 1: 95 nCPM
Immune cell
- myeloid DC: 34 nTPM
- classical monocyte: 29 nTPM
- non-classical monocyte: 21 nTPM
- intermediate monocyte: 15 nTPM
- plasmacytoid DC: 14 nTPM
- total PBMC: 11 nTPM
Brain region
- cerebral cortex: 73 nTPM
- hippocampal formation: 68 nTPM
- thalamus: 67 nTPM
- hypothalamus: 65 nTPM
- midbrain: 63 nTPM
- pons: 63 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.84
- gnomAD pLI
- 0.46
- gnomAD missense Z
- 1.13
- DepMap mean gene effect
- -0.11
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- cellular response to epidermal growth factor stimulus
- dephosphorylation
- negative regulation of cell migration
- negative regulation of chemotaxis
- negative regulation of epidermal growth factor receptor signaling pathway
- negative regulation of ERK1 and ERK2 cascade
- negative regulation of JNK cascade
- negative regulation of MAPK cascade
- negative regulation of T cell activation
- negative regulation of T cell receptor signaling pathway
- peptidyl-tyrosine dephosphorylation
- positive regulation of focal adhesion disassembly
- positive regulation of mitotic cell cycle
- regulation of focal adhesion assembly
Molecular functions
- cytoskeletal protein binding
- MAP kinase phosphatase activity
- phosphatase activity
- protein kinase binding
- protein serine/threonine phosphatase activity
- protein tyrosine kinase binding
- protein tyrosine phosphatase activity
- protein tyrosine/serine/threonine phosphatase activity
- receptor signaling protein tyrosine kinase inhibitor activity
- receptor tyrosine kinase binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Dual specificity phosphatase, catalytic domain
- Tyrosine-specific protein phosphatases domain
- Protein-tyrosine phosphatase, active site
- Atypical dual specificity phosphatase, subfamily A
- Dual specificity protein phosphatase domain
- Protein-tyrosine phosphatase-like
- Dual specificity phosphatase, catalytic domain
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of DUSP3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads DUSP3 as an antibody target. Whether an autoantibody or antibody against DUSP3 could matter depends on whether native DUSP3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
DUSP3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label DUSP3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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