DUS4L
tRNA-dihydrouridine(20a/20b) synthase [NAD(P)+]-like
Also known as: DUS4, DUS4L_HUMAN, PP35
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O95620
- Gene
- DUS4L
- Ensembl
- ENSG00000105865
- Chromosome
- 7
- Canonical length
- 317 aa
- Protein class
- Enzymes, Predicted intracellular proteins
- Subcellular location
- Cytosol
OverviewNCBI Gene
Predicted to enable tRNA dihydrouridine synthase activity. Involved in tRNA dihydrouridine synthesis. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
317 residues, UniProt reviewed canonical sequence.
>O95620|DUS4L
1 MKSDCMQTTI CQERKKDPIE MFHSGQLVKV CAPMVRYSKL AFRTLVRKYS CDLCYTPMIV
61 AADFVKSIKA RDSEFTTNQG DCPLIVQFAA NDARLLSDAA RIVCPYANGI DINCGCPQRW
121 AMAEGYGACL INKPELVQDM VKQVRNQVET PGFSVSIKIR IHDDLKRTVD LCQKAEATGV
181 SWITVHGRTA EERHQPVHYD SIKIIKENMS IPVIANGDIR SLKEAENVWR ITGTDGVMVA
241 RGLLANPAMF AGYEETPLKC IWDWVDIALE LGTPYMCFHQ HLMYMMEKIT SRQEKRVFNA
301 LSSTSAIIDY LTDHYGILocalizationUniProt · AlphaFold · HPA
Whether an antibody against DUS4L can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Unknown
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.24
- Highest tissue expression
- 3.4 nTPM
Expression across tissuesHPA
Tissue
- spinal cord: 3.4 nTPM
- midbrain: 2.8 nTPM
- amygdala: 2.7 nTPM
- basal ganglia: 2.6 nTPM
- cerebral cortex: 2.4 nTPM
- hypothalamus: 2.4 nTPM
Single-cell type
- oligodendrocytes: 13 nCPM
- distal convoluted tubule cells: 11 nCPM
- renal collecting duct intercalated cells: 11 nCPM
- renal collecting duct principal cells: 9.7 nCPM
- other brain neurons: 8.8 nCPM
- brain inhibitory neurons: 8.7 nCPM
Immune cell
- memory CD4 T-cell: 0.4 nTPM
- naive CD4 T-cell: 0.3 nTPM
- naive CD8 T-cell: 0.3 nTPM
- gdT-cell: 0.2 nTPM
- MAIT T-cell: 0.2 nTPM
- memory B-cell: 0.2 nTPM
Brain region
- white matter: 1.4 nTPM
- medulla oblongata: 1.2 nTPM
- cerebral cortex: 1.1 nTPM
- hypothalamus: 1.1 nTPM
- spinal cord: 1.1 nTPM
- basal ganglia: 1 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.11
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.75
- DepMap mean gene effect
- 0.01
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 13% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
- flavin adenine dinucleotide binding
- tRNA dihydrouridine synthase activity
- tRNA-dihydrouridine20a synthase activity
- tRNA-dihydrouridine20b synthase activity
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads DUS4L as an antibody target. Whether an autoantibody or antibody against DUS4L could matter depends on whether native DUS4L is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
DUS4L is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label DUS4L as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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