DUS2

Protein identityUniProt · HPA

UniProt accession

Q9NX74

Gene

DUS2

Ensembl

ENSG00000167264

Chromosome

16

Canonical length

493 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

This gene encodes a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. The resulting modified bases confer enhanced regional flexibility to tRNA. The encoded protein may increase the rate of translation by inhibiting an interferon-induced protein kinase. This gene has been implicated in pulmonary carcinogenesis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

493 residues, UniProt reviewed canonical sequence.

>Q9NX74|DUS2
     1  MILNSLSLCY HNKLILAPMV RVGTLPMRLL ALDYGADIVY CEELIDLKMI QCKRVVNEVL
    61  STVDFVAPDD RVVFRTCERE QNRVVFQMGT SDAERALAVA RLVENDVAGI DVNMGCPKQY
   121  STKGGMGAAL LSDPDKIEKI LSTLVKGTRR PVTCKIRILP SLEDTLSLVK RIERTGIAAI
   181  AVHGRKREER PQHPVSCEVI KAIADTLSIP VIANGGSHDH IQQYSDIEDF RQATAASSVM
   241  VARAAMWNPS IFLKEGLRPL EEVMQKYIRY AVQYDNHYTN TKYCLCQMLR EQLESPQGRL
   301  LHAAQSSREI CEAFGLGAFY EETTQELDAQ QARLSAKTSE QTGEPAEDTS GVIKMAVKFD
   361  RRAYPAQITP KMCLLEWCRR EKLAQPVYET VQRPLDRLFS SIVTVAEQKY QSTLWDKSKK
   421  LAEQAAAIVC LRSQGLPEGR LGEESPSLHK RKREAPDQDP GGPRAQELAQ PGDLCKKPFV
   481  ALGSGEESPL EGW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DUS2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

27 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.91

gnomAD pLI

0

gnomAD missense Z

0.6

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• antiviral innate immune response
• tRNA dihydrouridine synthesis

Molecular functions

• double-stranded RNA binding
• flavin adenine dinucleotide binding
• FMN binding
• NADPH binding
• protein kinase inhibitor activity
• tRNA binding
• tRNA dihydrouridine synthase activity
• tRNA-dihydrouridine20 synthase activity

Cellular components

• cytoplasm
• cytosol
• endoplasmic reticulum
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Aldolase-type TIM barrel
• Double-stranded RNA-binding domain
• tRNA-dihydrouridine synthase, conserved site
• DUS-like, FMN-binding domain
• Double-stranded RNA binding motif
• Dihydrouridine synthase (Dus)
• DUS2, double-stranded RNA binding domain
• tRNA-dihydrouridine synthase-like

KeywordsUniProt

• Cytoplasm
• Endoplasmic reticulum
• Flavoprotein
• FMN
• NADP
• Oxidoreductase
• Phosphoprotein
• RNA-binding
• tRNA processing

InteractionsUniProt · HPA

Protein binding partners of DUS2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• EPRS1