Seroatlas · Human Serome Atlas

DUOXA1

Dual oxidase maturation factor 1

Also known as: DOXA1_HUMAN, FLJ32334, mol, NIP, NUMBIP

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q1HG43
Gene: DUOXA1
Ensembl: ENSG00000140254
Chromosome: 15
Canonical length: 343 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

Dual oxidases DUOX1 and DUOX2 are NADPH oxidases which are involved in hydrogen peroxide production necessary for thyroid hormonogenesis. They form a heterodimer with specific maturation factors DUOXA1 and DUOXA2, respectively, which is essential for the maturation and function of the DUOX enzyme complexes. This gene encodes the DUOX1 activator or maturation factor DUOXA1. Rat studies identified a bidirectional promoter which controls the transcription of the DUOX1 and DUOXA1 genes. This protein is cotransported to the cell surface when coexpressed with DUOX1 and is retained in the endoplasmic reticulum when expressed without DUOX1 protein. The expression of this gene or the DUOX1 gene is not suppressed by thyroglobulin (Tg), a macromolecular precursor in thyroid hormone synthesis, while the expression of the DUOX2 and DUOXA2 are significantly suppressed by the Tg. This protein is also a p53-regulated neurogenic factor involved in p53 dependent neuronal differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2013]

Canonical amino-acid sequenceUniProt

343 residues, UniProt reviewed canonical sequence.

>Q1HG43|DUOXA1
     1  MATLGHTFPF YAGPKPTFPM DTTLASIIMI FLTALATFIV ILPGIRGKTR LFWLLRVVTS
    61  LFIGAAILAV NFSSEWSVGQ VSTNTSYKAF SSEWISADIG LQVGLGGVNI TLTGTPVQQL
   121  NETINYNEEF TWRLGENYAE EYAKALEKGL PDPVLYLAEK FTPRSPCGLY RQYRLAGHYT
   181  SAMLWVAFLC WLLANVMLSM PVLVYGGYML LATGIFQLLA LLFFSMATSL TSPCPLHLGA
   241  SVLHTHHGPA FWITLTTGLL CVLLGLAMAV AHRMQPHRLK AFFNQSVDED PMLEWSPEEG
   301  GLLSPRYRSM ADSPKSQDIP LSEASSTKAY CKEAHPKDPD CAL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DUOXA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 5
Mean surface accessibility (rSASA): 0.39
Highest tissue expression: 137 nTPM

Expression across tissuesHPA

Tissue

esophagus: 137 nTPM
thyroid gland: 79 nTPM
skin: 65 nTPM
vagina: 53 nTPM
lung: 47 nTPM
cervix: 43 nTPM

Single-cell type

esophageal apical cells: 388 nCPM
alveolar cells type 1: 219 nCPM
alveolar cells type 2: 216 nCPM
esophageal suprabasal cells: 180 nCPM
transitional alveolar cells: 155 nCPM
suprabasal keratinocytes: 100 nCPM

Immune cell

MAIT T-cell: 0.3 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM

Brain region

white matter: 6.6 nTPM
cerebral cortex: 3.2 nTPM
medulla oblongata: 2.5 nTPM
thalamus: 2.5 nTPM
cerebellum: 2.3 nTPM
pons: 2.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.98
gnomAD pLI: 0
gnomAD missense Z: 0.72
DepMap mean gene effect: -0.09
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

enzyme binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DUOXA1 as an antibody target. Whether an autoantibody or antibody against DUOXA1 could matter depends on whether native DUOXA1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DUOXA1 is annotated at the cell surface, where native DUOXA1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label DUOXA1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...