Seroatlas · Human Serome Atlas

DNPEP

Aspartyl aminopeptidase

Also known as: ASPEP, DAP, DNPEP_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9ULA0
Gene
DNPEP
Ensembl
ENSG00000123992
Chromosome
2
Canonical length
485 aa
Protein class
Enzymes, Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Cytosol
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene is an aminopeptidase which prefers acidic amino acids, and specifically favors aspartic acid over glutamic acid. It is thought to be a cytosolic protein involved in general metabolism of intracellular proteins. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Canonical amino-acid sequenceUniProt

485 residues, UniProt reviewed canonical sequence.

>Q9ULA0|DNPEP
     1  MSGHSPTRGA MQVAMNGKAR KEAVQTAAKE LLKFVNRSPS PFHAVAECRN RLLQAGFSEL
    61  KETEKWNIKP ESKYFMTRNS STIIAFAVGG QYVPGNGFSL IGAHTDSPCL RVKRRSRRSQ
   121  VGFQQVGVET YGGGIWSTWF DRDLTLAGRV IVKCPTSGRL EQQLVHVERP ILRIPHLAIH
   181  LQRNINENFG PNTEMHLVPI LATAIQEELE KGTPEPGPLN AVDERHHSVL MSLLCAHLGL
   241  SPKDIVEMEL CLADTQPAVL GGAYDEFIFA PRLDNLHSCF CALQALIDSC AGPGSLATEP
   301  HVRMVTLYDN EEVGSESAQG AQSLLTELVL RRISASCQHP TAFEEAIPKS FMISADMAHA
   361  VHPNYLDKHE ENHRPLFHKG PVIKVNSKQR YASNAVSEAL IREVANKVKV PLQDLMVRND
   421  TPCGTTIGPI LASRLGLRVL DLGSPQLAMH SIREMACTTG VLQTLTLFKG FFELFPSLSH
   481  NLLVD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNPEP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
64 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 64 nTPM
  • testis: 58 nTPM
  • duodenum: 53 nTPM
  • small intestine: 53 nTPM
  • tongue: 43 nTPM
  • heart muscle: 39 nTPM

Single-cell type

  • late spermatids: 716 nCPM
  • early spermatids: 467 nCPM
  • enterocytes: 206 nCPM
  • migrating cytotrophoblasts: 150 nCPM
  • decidual stromal cells: 139 nCPM
  • extravillous trophoblasts: 130 nCPM

Immune cell

  • NK-cell: 21 nTPM
  • myeloid DC: 21 nTPM
  • intermediate monocyte: 20 nTPM
  • MAIT T-cell: 19 nTPM
  • naive CD8 T-cell: 19 nTPM
  • memory CD8 T-cell: 18 nTPM

Brain region

  • thalamus: 23 nTPM
  • hypothalamus: 22 nTPM
  • spinal cord: 20 nTPM
  • hippocampal formation: 20 nTPM
  • medulla oblongata: 19 nTPM
  • basal ganglia: 19 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.93
gnomAD pLI
0
gnomAD missense Z
0.37
DepMap mean gene effect
0.03
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

  • Peptidase M18
  • Peptidase M18, domain 2
  • Aminopeptidase I zinc metalloprotease (M18)

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNPEP as an antibody target. Whether an autoantibody or antibody against DNPEP could matter depends on whether native DNPEP is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNPEP is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNPEP as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/DNPEP. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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