Seroatlas · Human Serome Atlas

DNAJC25

DnaJ homolog subfamily C member 25

Also known as: bA16L21.2.1, DJC25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H1X3
Gene: DNAJC25
Ensembl: ENSG00000059769
Chromosome: 9
Canonical length: 360 aa
Protein class: Predicted membrane proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Predicted to be involved in protein folding. Predicted to be located in membrane. Predicted to be active in endoplasmic reticulum membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

360 residues, UniProt reviewed canonical sequence.

>Q9H1X3|DNAJC25
     1  MGAPLLSPGW GAGAAGRRWW MLLAPLLPAL LLVRPAGALV EGLYCGTRDC YEVLGVSRSA
    61  GKAEIARAYR QLARRYHPDR YRPQPGDEGP GRTPQSAEEA FLLVATAYET LKDEETRKDY
   121  DYMLDHPEEY YSHYYHYYSR RLAPKVDVRV VILVSVCAIS VFQFFSWWNS YNKAISYLAT
   181  VPKYRIQATE IAKQQGLLKK AKEKGKNKKS KEEIRDEEEN IIKNIIKSKI DIKGGYQKPQ
   241  ICDLLLFQII LAPFHLCSYI VWYCRWIYNF NIKGKEYGEE ERLYIIRKSM KMSKSQFDSL
   301  EDHQKETFLK RELWIKENYE VYKQEQEEEL KKKLANDPRW KRYRRWMKNE GPGRLTFVDD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJC25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 3
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 30 nTPM

Expression across tissuesHPA

Tissue

liver: 30 nTPM
choroid plexus: 5.2 nTPM
adrenal gland: 4.9 nTPM
kidney: 4.7 nTPM
epididymis: 4.2 nTPM
parathyroid gland: 4 nTPM

Single-cell type

choroid plexus epithelial cells: 36 nCPM
ependymal cells: 36 nCPM
hepatocytes: 26 nCPM
brain excitatory neurons: 24 nCPM
brain inhibitory neurons: 24 nCPM
other brain neurons: 21 nCPM

Immune cell

eosinophil: 0.2 nTPM
intermediate monocyte: 0.2 nTPM
naive B-cell: 0.1 nTPM
neutrophil: 0.1 nTPM
non-classical monocyte: 0.1 nTPM
plasmacytoid DC: 0.1 nTPM

Brain region

choroid plexus: 4.5 nTPM
medulla oblongata: 2.1 nTPM
midbrain: 1.7 nTPM
basal ganglia: 1.6 nTPM
cerebral cortex: 1.6 nTPM
hippocampal formation: 1.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.07
gnomAD pLI: 0
gnomAD missense Z: 0.93
DepMap mean gene effect: 0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein folding

Cellular components

endoplasmic reticulum membrane

Protein domainsUniProt · Pfam · InterPro

DnaJ domain
Chaperone J-domain superfamily
DnaJ domain
DnaJ homolog subfamily C member 25-like

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJC25 as an antibody target. Whether an autoantibody or antibody against DNAJC25 could matter depends on whether native DNAJC25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJC25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJC25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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