Seroatlas · Human Serome Atlas

DNAJC24

DnaJ homolog subfamily C member 24

Also known as: DJC24_HUMAN, DPH4, JJJ3, ZCSL3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6P3W2
Gene: DNAJC24
Ensembl: ENSG00000170946
Chromosome: 11
Canonical length: 149 aa
Protein class: Predicted intracellular proteins
Subcellular location: Cytosol
Quaternary structure: Homooligomer

OverviewNCBI Gene

Diphthamide is a unique posttranslationally modified histidine found only in translation elongation factor-2 (EEF2; MIM 130610). This modification is conserved from archaebacteria to humans and serves as the target for ADP-ribosylation and inactivation of EEF2 by diphtheria toxin (DT) and Pseudomonas exotoxin A. DPH4 is 1 of several enzymes involved in synthesis of diphthamide in EEF2 (Liu et al., 2004 [PubMed 15485916]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

149 residues, UniProt reviewed canonical sequence.

>Q6P3W2|DNAJC24
     1  MMAVEQMPKK DWYSILGADP SANISDLKQK YQKLILMYHP DKQSTDVPAG TVEECVQKFI
    61  EIDQAWKILG NEETKREYDL QRCEDDLRNV GPVDAQVYLE EMSWNEGDHS FYLSCRCGGK
   121  YSVSKDEAEE VSLISCDTCS LIIELLHYN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DNAJC24 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.4
Highest tissue expression: 16 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 16 nTPM
heart muscle: 11 nTPM
tongue: 11 nTPM
cerebellum: 9.6 nTPM
hypothalamus: 8.9 nTPM
hippocampal formation: 8.5 nTPM

Single-cell type

late spermatids: 356 nCPM
early spermatids: 219 nCPM
late primary spermatocytes: 139 nCPM
somatotrophs: 89 nCPM
corticotrophs: 85 nCPM
thyrotrophs: 81 nCPM

Immune cell

basophil: 4.4 nTPM
MAIT T-cell: 3.1 nTPM
naive CD8 T-cell: 2.9 nTPM
naive CD4 T-cell: 2.8 nTPM
gdT-cell: 2.2 nTPM
memory CD4 T-cell: 2.1 nTPM

Brain region

cerebellum: 15 nTPM
hypothalamus: 14 nTPM
midbrain: 13 nTPM
hippocampal formation: 12 nTPM
medulla oblongata: 12 nTPM
thalamus: 12 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.82
gnomAD pLI: 0
gnomAD missense Z: 0.66
DepMap mean gene effect: 0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

actin cytoskeleton

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads DNAJC24 as an antibody target. Whether an autoantibody or antibody against DNAJC24 could matter depends on whether native DNAJC24 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

DNAJC24 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label DNAJC24 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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